Počet záznamů: 1
Leishmania in sand flies: comparison of quantitative polymerase chain reaction with other techniques to determine the intensity of infection
- 1.
SYSNO ASEP 0318321 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Leishmania in sand flies: comparison of quantitative polymerase chain reaction with other techniques to determine the intensity of infection Překlad názvu Leishmania ve flebotomech: srovnání kvantitativní PCR s ostatními technikami kvantifikace infekce Tvůrce(i) Myšková, J. (CZ)
Votýpka, Jan (BC-A) RID, ORCID
Volf, P. (CZ)Celkový počet autorů 3 Zdroj.dok. Journal of Medical Entomology. - : Oxford University Press - ISSN 0022-2585
Roč. 45, č. 1 (2008), s. 133-138Poč.str. 6 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova sand fly ; Leishmania ; Phlebotomus ; parasite-vector interaction ; RT-PCR Vědní obor RIV EG - Zoologie CEP LC06009 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy CEZ AV0Z60220518 - PAU-O, BC-A (2005-2011) UT WOS 000252635500020 Anotace We compared quantitative polymerase chain reaction (Q-PCR) with two traditional methods, estimation in situ and direct counting with the aid of a hemocytometer, to evaluate their usefulness. Phlebotomus duboscqi and Ph. arabicus were infected with Leishmania major and L. infantum, respectively, and different approaches were compared to determine the intensity of Leishmania infections before and after defecation of the bloodmeal. Estimation of parasite numbers in situ is only a semiquantitative method, but it is quick and provides data about localization of infection. We recommend this technique for low-intensity infections after the bloodmeal is passed. Counting in a hemocytometer is a suitable technique for heavily infected sand flies or for quantification of Leishmania within the bloodmeal. The most accurate approach for parasite quantification in any type of sand fly infection is Q-PCR. This method is also highly sensitive and can detect one parasite per gut. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2009
Počet záznamů: 1