Počet záznamů: 1

Fluoroalkyl chloroformates in treating amino acids for gas chromatographic analysis

SYSNO ASEP	0305774
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Ostatní články
Název	Fluoroalkyl chloroformates in treating amino acids for gas chromatographic analysis
Překlad názvu	Derivatizace aminokyselin fluoralkychlormravenčany pro analýzy plynovou chromatografií
Tvůrce(i)	Hušek, Petr (BC-A) Šimek, Petr (BC-A)_{RID, ORCID} Hartvich, Petr (BC-A) Zahradníčková, Helena (BC-A)_{RID, ORCID}
Zdroj.dok.	Journal of Chromatography A. - : Elsevier - ISSN 0021-9673 Roč. 1186, 1/2 (2008), s. 391-400
Poč.str.	10 s.
Jazyk dok.	eng - angličtina
Země vyd.	NL - Nizozemsko
Klíč. slova	amino acids ; derivatization ; pentafluoropropyl- and heptafluorobutyl chloroformates
Vědní obor RIV	CB - Analytická chemie, separace
CEP	GA203/04/0192 GA ČR - Grantová agentura ČR
	GA303/06/1674 GA ČR - Grantová agentura ČR
CEZ	AV0Z50070508 - ENTU-I, BC-A (2005-2011)
DOI	10.1016/j.chroma.2007.11.117
Anotace	Novel fluoroalkyl chloroformates with 3 and 4 carbon atoms were investigated to immediate conversion of amino acids into hydrophobic derivatives in water-containing media. Derivatization conditions were extensively studied and optimized sample preparation protocols elaborated. More than 30 amino acids were treated with the particular reagent in isooctane by simply vortexing the reactive organic phase with a slightly basified aqueous medium containing pyridine or 3-picoline as a catalyst. Outstanding separation of nearly all components on 5% phenylmethylsilicone phase in gas chromatographic (GC) analysis with mass spectrometric (MS) or flame ionization detection (FID) required <10 min. Quantitation characteristics involving linearity in range of 0.1 to 100 nmol, MS limit of detection (LOD, 0.03 pmol - 20 pmol) and electron impact (EI) spectra and diagnostic SIM fragment ions of the derivatives are reported. The novel method is simple, robust and rapid, running in aqueous environment.
Pracoviště	Biologické centrum (od r. 2006)
Kontakt	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Rok sběru	2008

Počet záznamů: 1