Počet záznamů: 1
Bilayer Localization of Membrane-Active Peptides Studied in Biomimetic Vesicles by Visible and Fluorescence Spectroscopies
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SYSNO ASEP 0181772 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Ostatní články Název Bilayer Localization of Membrane-Active Peptides Studied in Biomimetic Vesicles by Visible and Fluorescence Spectroscopies Tvůrce(i) Sheynis, T. (IL)
Sýkora, Jan (UFCH-W) RID
Benda, Aleš (UFCH-W) RID, ORCID
Kolusheva, S. (IL)
Hof, Martin (UFCH-W) RID, ORCID
Jelinek, R. (IL)Zdroj.dok. European Journal of Biochemistry - ISSN 0014-2956
Roč. 270, č. 22 (2003), s. 4478-4487Poč.str. 10 s. Jazyk dok. eng - angličtina Země vyd. DE - Německo Klíč. slova solvent relaxation ; fluorescence correlation spectroscopy ; lipid bilayers Vědní obor RIV CF - Fyzikální chemie a teoretická chemie CEP LN00A032 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy CEZ AV0Z4040901 - UFCH-W Anotace Depth of bilayer penetration and effects on lipid mobility conferred by the membrane-active peptides magainin, melittin, and a hydrophobic helical sequence KKA(LA)(7)KK (denoted KAL), were investigated by colorimetric and time-resolved fluorescence techniques in biomimetic phospholipid/poly(diacetylene) vesicles. The experiments demonstrated that the extent of bilayer permeation and peptide localization within the membrane was dependent upon the bilayer composition, and that distinct dynamic modifications were induced each peptide within the head-group environment of the phospholipids. Solvent relaxation, fluorescence correlation spectroscopy and fluorescence quenching analyses, employing probes at different locations within the bilayer, showed that magainin and melittin inserted close to the glycerol residues in bilayers incorporating negatively charged phospholipids, but predominant association at the lipid-water interface occurred in bilayers containing zwitterionic phospholipids. Pracoviště Ústav fyzikální chemie J.Heyrovského Kontakt Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196 Rok sběru 2004
Počet záznamů: 1