Počet záznamů: 1
Transient accumulation of cis- and trans-zeatin type cytokinins and its relation to cytokinin oxidase activity during cell cycle of synchronized tobacco BY-2 cells
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SYSNO ASEP 0172240 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Ostatní články Název Transient accumulation of cis- and trans-zeatin type cytokinins and its relation to cytokinin oxidase activity during cell cycle of synchronized tobacco BY-2 cells Tvůrce(i) Dobrev, Petre (UEB-Q) RID, ORCID
Motyka, Václav (UEB-Q) RID, ORCID
Gaudinová, Alena (UEB-Q) ORCID
Malbeck, Jiří (UEB-Q) RID, ORCID
Trávníčková, Alena (UEB-Q) ORCID
Kamínek, Miroslav (UEB-Q) RID, ORCID
Vaňková, Radomíra (UEB-Q) RID, ORCIDZdroj.dok. Plant Physiology and Biochemistry. - : Elsevier - ISSN 0981-9428
Roč. 40, - (2002), s. 333-337Poč.str. 5 s. Jazyk dok. eng - angličtina Země vyd. CZ - Česká republika Klíč. slova Cis zeatin ; Cytokinins ; Cytokinin oxidase Vědní obor RIV EB - Genetika a molekulární biologie CEP GA522/99/1130 GA ČR - Grantová agentura ČR GV206/96/K188 GA ČR - Grantová agentura ČR IAA6038002 GA AV ČR - Akademie věd CEZ AV0Z5038910 - UEB-Q Anotace Changes in levels of endogenous cytokinins and activity of cytokinin oxidase (EC 1.4.3.6) were followed during the cell cycle progression in large-scale (ca. 30 g FW) synchronized culture of the tobacco cell line BY-2. Synchronization was induced either by 4 mM hydroxyurea or 15 iM aphidicolin alone (a single block of the cell cycle at G,/S) or in combination with 1 uM oryzalin (double blocking at G,/S and Gz/M). Up to 46% synchrony was obtained. Two distinct cytokinin peaks were detected at 0-2 h and 8.5-9 h after the release from the first block coinciding with the beginning of the S phase and with the onset of mitosis, respectively. Interestingly, as identified by MS/MS, very sharp and transient premitotic peak of cytokinins contained, in addition to fra/u-zeatin, also considerable amounts of cu-zeatin and zeatin-0-glucoside. Cytokinin oxidase activity exhibited conspicuous maximum parallel to the first peak of cytokinin accumulation and deep minimum corresponding to the culmination of mitotic index. Possible roles of zeatin cis-trans isomerisation, glucosylation and degradation by cytokinin oxidase in regulation of cytokinin levels during the cell cycle progression are discussed. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2003
Počet záznamů: 1