Počet záznamů: 1
Fast sex identification in wild mammals using PCR amplification of the Sry gene
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SYSNO ASEP 0164504 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Fast sex identification in wild mammals using PCR amplification of the Sry gene Tvůrce(i) Bryja, Josef (UBO-W) RID, SAI, ORCID
Konečný, A. (CZ)Zdroj.dok. Folia zoologica. - : Ústav biologie obratlovců AV ČR, v. v. i. - ISSN 0139-7893
Roč. 52, č. 3 (2003), s. 269-274Poč.str. 6 s. Jazyk dok. eng - angličtina Země vyd. CZ - Česká republika Klíč. slova sex identification ; Sry gene ; rodents Vědní obor RIV EG - Zoologie CEP GA524/01/1316 GA ČR - Grantová agentura ČR GP206/02/P068 GA ČR - Grantová agentura ČR CEZ AV0Z6093917 - UBO-W UT WOS 000185864800005 EID SCOPUS 0142091382 Anotace Several primer sets were described for PCR amplification of the Sry gene that became very suitable method for sex identification in mammals. We tested applicability of two primer sets for sex identification in one bat and 12 rodent species from Central Europe. Rapid DNA extraction with non-ionic detergents, which is very suitable for large-scale population studies, was used. Primers SRY-A from Pomp et al. (1995) did not amplify Sry fragment in males of Myotis myotis, four species of Microtus, and Clethrionomys glareolus, while amplification was successful in four species of Apodemus, two species of Mus, and Sciurus vulgaris. On the other hand, primers from Sanchez et al. (1996) gave clear Sry band in males of all tested species, only with one exception, i.e. male of Sciurus vulgaris. Pracoviště Ústav biologie obratlovců Kontakt Hana Slabáková, slabakova@ivb.cz, Tel.: 543 422 524 Rok sběru 2004
Počet záznamů: 1