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Expansion during PCR of short single-stranded DNA fragments carrying nonselfcomplementary dinucleotide or trinucleotide repeats

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    SYSNO ASEP0127065
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    Poddruh JOstatní články
    NázevExpansion during PCR of short single-stranded DNA fragments carrying nonselfcomplementary dinucleotide or trinucleotide repeats
    Tvůrce(i) Reichová, Naďa (BFU-R)
    Kypr, Jaroslav (BFU-R) RID
    Zdroj.dok.Molecular Biology Reports - ISSN 0301-4851
    Roč. 30, č. 3 (2003), s. 155-163
    Poč.str.9 s.
    Jazyk dok.eng - angličtina
    Země vyd.NL - Nizozemsko
    Klíč. slovaDNA ; PCR ; expansion
    Vědní obor RIVBO - Biofyzika
    CEPGA301/01/0590 GA ČR - Grantová agentura ČR
    CEZAV0Z5004920 - BFU-R
    AnotaceWe performed PCR of many DNA fragments of 6-32 nucleotides in length. Some of the fragments expanded into kilobase lengths even in the absence of the complementary strand. The dramatic expansion was observed for (CA)(8), (TG)(8), (CA)(4), (CA)(6), (CA)(12), (TG)(4), (TG)(6), (TG)(12), (TC)(10), (GA)(10) and other single strands. Similar expansions were exhibited by related trinucleotide repeats (TTG)(5), (CAA)(5), (TGG)(5), and (CCA)(5) as well. However even small perturbations of the strict repetitive nature of the DNA primary structure substantially reduced the expansions. The expansion products had properties characteristic for normal Watson-Crick duplexes. Hence either the Taq polymerase and/or other components of the PCR buffer promote homoduplex formation of the non-selfcomplementary fragments, which is necessary to prime the synthesis of the complementary DNA strand, or the Taq polymerase is able to copy the single-stranded DNA template without any priming effect.
    PracovištěBiofyzikální ústav
    KontaktJana Poláková, polakova@ibp.cz, Tel.: 541 517 244
    Rok sběru2004

Počet záznamů: 1