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Novel cationic transport agents for oligonucleotide delivery into primary leukemic cells
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SYSNO ASEP 0108989 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Ostatní články Název Novel cationic transport agents for oligonucleotide delivery into primary leukemic cells Překlad názvu Nové kationtové transportní látky pro přenos oligonukleotidů do primárních leukemických buněk Tvůrce(i) Králová, Jarmila (UMG-J) RID
Dvořák, Michal (UMG-J) RID
Král, V. (CZ)Zdroj.dok. Journal of Medicinal Chemistry. - : American Chemical Society - ISSN 0022-2623
Roč. 46, č. 11 (2003), s. 2049-2056Poč.str. 8 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova oligonucleotide delivery ; primary leukemic cells ; antisense effect Vědní obor RIV EB - Genetika a molekulární biologie CEP GV301/98/K042 GA ČR - Grantová agentura ČR GA301/01/0976 GA ČR - Grantová agentura ČR GA203/02/0420 GA ČR - Grantová agentura ČR CEZ AV0Z5052915 - UMG-J Anotace The main aim of this work was to elaborate the delivery system for oligonucleotide (ODN into primary leukemic cells. Novel cationic compounds forming complexes with ODN were prepared, and their transporting ability tested. Two cationic porphyrin derivatives (2 and 3) were found to be at least 1 order of magnitude more efficient in this respect than commercially available agents. The ODN transporting capacity of novel compounds was dependent on the magnitude and the nature of their positive charges as well as on the porphyrin/ODN molar ratio. Porphyrin-ODN complexes were internalized into cells, and their dissociation was demonstrated by accumulation of fluorescein isothiocyanate-ODN fluorescence in the nucleus. Importantly, porphyrin 3 significantly protected complexed ODN against degradation and efficiently mediated the specific antisense effect on targeted v-Myb expression, resulting in reproducible growth inhibition of treated cells. Low toxicity, serum compatibility, and water solubility of porphyrin 3 make this compound a promising novel tool for modulation of gene expression in primary leukemic cells Pracoviště Ústav molekulární genetiky Kontakt Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Rok sběru 2005
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