Počet záznamů: 1

Monitoring development of chemoresistance to cyclin-dependent kinases inhibition by two-dimensional liquid phase protein fractionation

SYSNO ASEP	0042104
Druh ASEP	C - Konferenční příspěvek (mezinárodní konf.)
Zařazení RIV	D - Článek ve sborníku
Název	Monitoring development of chemoresistance to cyclin-dependent kinases inhibition by two-dimensional liquid phase protein fractionation
Překlad názvu	Sledování vzniku chemorezistence k inhibici cyklin-dependentních kináz pomocí dvojrozměrné kapalinové separace proteinů
Tvůrce(i)	Skalníková, Helena (UZFG-Y)_{RID, ORCID} Halada, Petr (MBU-M)_{RID, ORCID} Hajdúch, M. (CZ) Vydra, D. (CZ) Dzubak, P. (CZ) Dziechciarková, M. (CZ) Strnad, M. (CZ) Kovářová, Hana (UZFG-Y)_{RID, ORCID}
Zdroj.dok.	Third Annual European Symposium on Protein Fractionation and Biomarker Discovery. - Oxford : Worcester College, 2006
Rozsah stran	s. 1
Poč.str.	1 s.
Akce	Third Annual European Symposium on Protein Fractionation and Biomarker Discovery
Datum konání	12.09.2006-13.09.2006
Místo konání	Oxford
Země	GB - Velká Británie
Typ akce	WRD
Jazyk dok.	eng - angličtina
Země vyd.	GB - Velká Británie
Klíč. slova	cyclin-dependent kinases
Vědní obor RIV	EB - Genetika a molekulární biologie
CEP	GA301/05/0418 GA ČR - Grantová agentura ČR
CEZ	AV0Z50450515 - UZFG-Y (2005-2011)
	AV0Z50200510 - MBU-M (2005-2011)
Anotace	Cyclin-dependent kinases (CDKs) are key regulators of cell cycle, differentiation and apoptosis. Deregulation of CDKs in many cancer cells has motivated the effort to develop pharmacologically active CDK inhibitors. In this study, we used proteomic approach to monitor response of T-lymphoblastic leukaemia CEM cell line to synthetic CDKs inhibitor, Bohemine. We have compared Bohemine effects on CEM line susceptible to this drug and on a Bohemine resistant cell line derived from CEM line by culture in presence of sub-lethal Bohemine doses. Proteins were separated by two-dimensional liquid chromatography PF 2D system (Beckman Coulter) using chromatofocusing in the first dimension followed by reversed phase HPLC. Changes in protein composition after Bohemine treatment of sensitive and resistant CEM line were evaluated and differently expressed proteins were selected for identification by mass spectrometry. In susceptible cells, down-regulation of three histone variants in response to CDKs inhibition indicated that anti-cancer activity of Bohemine may be associated with epigenetic regulation. Futhermore, down-regulation of Crk-like scaffold adaptor protein was found and verified by immunoblot and immunohistochemistry for in vitro and in vivo drug treatment, respectively. Crk-like protein represents a new important target of CDK inhibition in T-lymphoblastic leukaemia. Response of resistant cells was associated with increase in the level of many chaperones/stress proteins.
Pracoviště	Ústav živočišné fyziologie a genetiky
Kontakt	Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554
Rok sběru	2007

Počet záznamů: 1