Nanodiamonds as Effective Vectors for Micro RNA-Based Drug Delivery

0499815 - MBÚ 2019 RIV US eng J - Článek v odborném periodiku
Benson, Veronika - Neuhoferová, Eva
Nanodiamonds as Effective Vectors for Micro RNA-Based Drug Delivery.
Drug Designing & Intellectual Properties International Journal. Roč. 1, č. 4 (2018), s. 112-117. ISSN 2637-4706
Grant CEP: GA MZd(CZ) NV15-33094A
Institucionální podpora: RVO:61388971
Klíčová slova: Micro-RNA * RNA interference * Nano Diamond
Obor OECD: Microbiology

Micro RNAs are short non-coding RNA molecules, which are important regulators of cell functions. Deregulation of certain micro RNA level is contributing factor to tumor formation and progression. Possible gene therapy by modulation of the micro RNA level is currently considered. Recently it has been proved that nano diamonds coated with PEI serve as efficient transfection and monitoring system. The aim of our work was to deliver micro RNA inhibitor and micro RNA mimic sequences into specialized mammalian
cells using fluorescent nano diamond particles and to test the nano complex uptake and cell compatibility during primary in vivo application. Short RNA molecules were bound to the PEI coated nano diamonds, transported into the colon cancer cell line in vitro and observed in a confocal microscope. The miRNA mimic coated nano diamonds were used for complex uptake and toxicity study in peritoneal macrophages and splenocytes after ex vivo stimulation or in vivo administration. Due to the possibility of continuous
monitoring of carrier internalization, its transfection efficacy, and cell compatibility, the fluorescent nano diamonds represent a suitable RNA carrier with a potential use in gene therapy.
Trvalý link: http://hdl.handle.net/11104/0292005