Počet záznamů: 1
Evaluation of basic mitochondrial functions using rat tissue homogenates
- 1.0369964 - FGÚ 2012 RIV NL eng J - Článek v odborném periodiku
Pecinová, Alena - Drahota, Zdeněk - Nůsková, Hana - Pecina, Petr - Houštěk, Josef
Evaluation of basic mitochondrial functions using rat tissue homogenates.
Mitochondrion. Roč. 11, č. 5 (2011), s. 722-728. ISSN 1567-7249. E-ISSN 1872-8278
Grant CEP: GA MZd(CZ) NS9759; GA ČR(CZ) GAP303/11/0970; GA MŠMT(CZ) 1M0520; GA MŠMT OC08017
Výzkumný záměr: CEZ:AV0Z50110509
Klíčová slova: oxidative phosphorylation * isolated mitochondria * tissue homogenates * respiratory control * membrane potential
Kód oboru RIV: FB - Endokrinologie, diabetologie, metabolizmus, výživa
Impakt faktor: 3.615, rok: 2011
The analysis of rat heart, liver, brain and kidney by oxygraphy, enzyme activities, membrane potential and BN/SDS-PAGE Western blotting demonstrated that tissue homogenates can fully substitute for isolated mitochondria, providing comparable qualitative mitochondrial parameters. The use of homogenate avoids the loss of the majority of mitochondria during their isolation. Only 50–100 mg of the tissue are required for the complex OXPHOS analysis
Trvalý link: http://hdl.handle.net/11104/0203900
Počet záznamů: 1