Počet záznamů: 1
Novel antimicrobial peptides from the venom of the eusocial bee Halictus sexcinctus (Hymenoptera: Halictidae) and their analogs
- 1.0351367 - ÚOCHB 2011 RIV AT eng J - Článek v odborném periodiku
Monincová, Lenka - Buděšínský, Miloš - Slaninová, Jiřina - Hovorka, Oldřich - Cvačka, Josef - Voburka, Zdeněk - Fučík, Vladimír - Borovičková, Lenka - Bednárová, Lucie - Straka, J. - Čeřovský, Václav
Novel antimicrobial peptides from the venom of the eusocial bee Halictus sexcinctus (Hymenoptera: Halictidae) and their analogs.
Amino Acids. Roč. 39, č. 3 (2010), s. 763-775. ISSN 0939-4451. E-ISSN 1438-2199
Grant CEP: GA ČR GA203/08/0536
Výzkumný záměr: CEZ:AV0Z40550506
Klíčová slova: antimicrobial peptides * Wild-bee venom * hemolytic activity * NMR spectroscopy * CD spectroscopy
Kód oboru RIV: CC - Organická chemie
Impakt faktor: 4.106, rok: 2010
Two novel antimicrobial peptides Gly-Met-Trp-Ser-Lys-Ile-Leu-Gly-His-Leu-Ile-Arg-NH2 (HAL-1) and Gly-Lys-Trp-Met-Ser-Leu-Leu-Lys-His-Ile-Leu-Lys-NH2 (HAL-2) designated halictines were identified in the venom of the wild bee Halictus sexcinctus. They possess high antimicrobial activity against both Gram-positive and -negative bacteria and noticeable hemolytic activity. As indicated by CD and NMR spectroscopy, halictines can adopt an amphipathic alpha-helical secondary structure in an anisotropic environment such as the bacterial cell membrane. Altogether we prepared 51 of HAL-1 and HAL-2 analogs to study the effect of such structural parameters as cationicity, hydrophobicity, alpha-helicity, amphipathicity, and truncation on antimicrobial and hemolytic activities.
Trvalý link: http://hdl.handle.net/11104/0191135
Počet záznamů: 1