Počet záznamů: 1
Crystallization and preliminary X-ray analysis of a novel haloalkane dehalogenase DbeA from Bradyrhizobium elkani USDA94
- 1.0343421 - ÚOCHB 2011 RIV GB eng J - Článek v odborném periodiku
Prudnikova, T. - Mozga, T. - Řezáčová, Pavlína - Chaloupková, R. - Sato, Y. - Nagata, Y. - Brynda, Jiří - Kutý, Michal - Damborský, J. - Kutá-Smatanová, Ivana
Crystallization and preliminary X-ray analysis of a novel haloalkane dehalogenase DbeA from Bradyrhizobium elkani USDA94.
Acta Crystallographica Section F-Structural Biology and Crystallization Communications. F65, č. 4 (2009), s. 353-356. ISSN 1744-3091. E-ISSN 2053-230X
Grant CEP: GA MŠMT(CZ) LC06010
Výzkumný záměr: CEZ:AV0Z40550506; CEZ:AV0Z60870520; CEZ:AV0Z50520514
Klíčová slova: protein crystallization * X-ray analysis * dehalogenase
Kód oboru RIV: CC - Organická chemie
Impakt faktor: 0.551, rok: 2009
A novel enzyme, DbeA, belonging to the haloalkane dehalogenase family (EC 3.8.1.5) was isolated from Bradyrhizobium elkani USDA94. In order to understand the unique activity and specificity of DbeA, its mutant variant DbeA1, which carries the unique fragment of DbjA, was also constructed. Both wild-type DbeA and DbeA1 were crystallized using the sitting-drop vapour-diffusion method. The crystals of DbeA belonged to the primitive orthorhombic space group P2(1)2(1)2(1), while the crystals of DbeA1 belonged to the monoclinic space group C2. Diffraction data were collected to 2.2 A resolution for both DbeA and DbeA1 crystals.
Trvalý link: http://hdl.handle.net/11104/0185903
Počet záznamů: 1