The Noncanonical Gag Domains p8 and n Are Critical for Assembly and Release of Mouse Mammary Tumor Virus

0349410 - ÚOCHB 2011 RIV US eng J - Článek v odborném periodiku
Zábranský, Aleš - Hoboth, Peter - Hadravová, Romana - Štokrová, Jitka - Sakalian, M. - Pichová, Iva
The Noncanonical Gag Domains p8 and n Are Critical for Assembly and Release of Mouse Mammary Tumor Virus.
Journal of Virology. Roč. 84, č. 21 (2010), s. 11555-11559. ISSN 0022-538X. E-ISSN 1098-5514
Grant CEP: GA MŠMT 1M0508
Výzkumný záměr: CEZ:AV0Z40550506
Klíčová slova: betaretrovirus * Gag * assembly
Kód oboru RIV: CE - Biochemie
Impakt faktor: 5.189, rok: 2010

The mouse mammary tumor virus (MMTV) Gag contains the unique domains pp21, p3, p8 and n. We investigated the contribution of these domains to particle assembly and found that the region spanning the p8 and n domains is critical for shape determination and assembly. Deletion of pp21 and p3 reduced the number of released particles, but deletion of the n domain resulted in frequent formation of aberrant particles, while deletion of p8 severely impaired assembly. Further investigation of the p8 revealed that both the basic and the proline-rich motifs within p8 contribute to MMTV assembly.
Trvalý link: http://hdl.handle.net/11104/0189661