0498894 - ÚŽFG 2019 RIV BG eng J - Článek v odborném periodiku
Hnátková, Eva - Triantaphyllidis, C. - Ozouf-Costaz, C. - Choleva, Lukáš - Majtánová, Zuzana - Bohlen, Jörg - Ráb, Petr
Karyotype and chromosomal characteristics of rDNA of Cobitis strumicae Karaman, 1955 (Teleostei, Cobitidae) from Lake Volvi, Greece.
Comparative Cytogenetics. Roč. 12, č. 4 (2018), s. 483-491. ISSN 1993-0771. E-ISSN 1993-078X
Grant CEP: GA MŠMT EF15_003/0000460; GA ČR(CZ) GA17-09807S
Institucionální podpora: RVO:67985904
Klíčová slova: chromosome banding * NOR phenotype * FISH
Obor OECD: Genetics and heredity (medical genetics to be 3)
Impakt faktor: 0.882, rok: 2018

The karyotype of Greek cobitid fish Cobitis strumicae Karaman, 1955, from Lake Volvi, Greece, a representative of one of its two major intraspecific phylogenetic lineages, was analysed by means of sequential Giemsa-staining, C-banding, silver-staining, CMA(3) fluorescence banding and also by in situ hybridization (FISH) with rDNA probe. The diploid chromosome number was 2n = 50, karyotype composed of 10 pairs of metacentric to submetacentric and 15 pairs of subtelocentric to acrocentric chromosomes. The nucleolus organizer regions (NORs) as revealed by Ag- and CMA(3) staining and FISH were situated in the telomeric region of the fourth submetacentric chromosome pair. The chromosomes contained very low content of C-positive heterochromatin. No heteromorphic sex chromosomes were detected. This first karyotype report for any species of lineage Bicanestrinia Bacescu, 1962 shows a simple karyotype dominated by acrocentric chromosomes and possessing single NOR-bearing chromosome pair. Cytotaxonomic implications of this finding for the taxonomy of the genus Cobitis Linnaeus, 1758 are further discussed.
Trvalý link: http://hdl.handle.net/11104/0291182