Comparative genomics of Czech vaccine strains of Bordetella pertussis.

0497620 - MBÚ 2019 RIV GB eng J - Článek v odborném periodiku
Dienstbier, Ana - Pouchnik, D. - Wildung, M. - Amman, F. - Hofacker, I.L. - Parkhill, J. - Holubová, Jana - Šebo, Peter - Večerek, Branislav
Comparative genomics of Czech vaccine strains of Bordetella pertussis.
Pathogens and Disease. Roč. 76, č. 7 (2018), č. článku fty071. ISSN 2049-632X. E-ISSN 2049-632X
Grant CEP: GA MZd(CZ) NV16-30782A
Grant ostatní: AV ČR(CZ) MSM200201702
Program: Program na podporu mezinárodní spolupráce začínajících výzkumných pracovníků
Institucionální podpora: RVO:61388971
Klíčová slova: Bordetella pertussis * genome rearrangement * vaccine pressure
Obor OECD: Microbiology
Impakt faktor: 2.182, rok: 2018

Bordetella pertussis is a strictly human pathogen causing the respiratory infectious disease called whooping cough or pertussis. B. pertussis adaptation to acellular pertussis vaccine pressure has been repeatedly highlighted, but recent data indicate that adaptation of circulating strains started already in the era of the whole cell pertussis vaccine (wP) use. We sequenced the genomes of five B. pertussis wP vaccine strains isolated in the former Czechoslovakia in the pre-wP (1954–1957) and early wP (1958–1965) eras, when only limited population travel into and out of the country was possible. Four isolates exhibit a similar genome organization and form a distinct phylogenetic cluster with a geographic signature. The fifth strain is rather distinct, both in genome organization and SNP-based phylogeny. Surprisingly, despite isolation of this strain before 1966, its closest sequenced relative appears to be a recent isolate from the US. On the genome content level, the five vaccine strains contained both new and already described regions of difference. One of the new regions contains duplicated genes potentially associated with transport across the membrane. The prevalence of this region in recent isolates indicates that its spread might be associated with selective advantage leading to increased strain fitness.
Trvalý link: http://hdl.handle.net/11104/0290168