Počet záznamů: 1
Nanodiamonds as „artificial proteins“: Regulation of a cell signalling system using low nanomolar solutions of inorganic nanocrystals
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SYSNO ASEP 0492357 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Nanodiamonds as „artificial proteins“: Regulation of a cell signalling system using low nanomolar solutions of inorganic nanocrystals Tvůrce(i) Bálek, L. (CZ)
Buchtová, Marcela (UZFG-Y) RID, ORCID
Kunová Bosáková, M. (CZ)
Vařecha, M. (CZ)
Foldynová-Trantírková, Silvie (BFU-R) ORCID
Gudernová, I. (CZ)
Veselá, I. (CZ)
Havlík, Jan (UOCHB-X) RID
Neburková, Jitka (UOCHB-X) RID, ORCID
Turner, S. (BE)
Krzyscik, M. A. (PL)
Zakrzewska, M. (PL)
Klimaschewski, L. (AT)
Claus, P. (DE)
Trantírek, L. (CZ)
Cígler, Petr (UOCHB-X) RID, ORCID
Krejčí, Pavel (UZFG-Y) ORCIDZdroj.dok. Biomaterials. - : Elsevier - ISSN 0142-9612
Roč. 176, SEP (2018), s. 106-121Poč.str. 16 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova nanodiamonds ; cell signalling ; FGF ; fibroblast growth factor ; nanotherapeutics Vědní obor RIV JJ - Ostatní materiály Obor OECD Nano-materials (production and properties) Vědní obor RIV – spolupráce Biofyzikální ústav - Ostatní materiály CEP EF15_003/0000477 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy GA17-09525S GA ČR - Grantová agentura ČR GA17-12075S GA ČR - Grantová agentura ČR EF16_019/0000729 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora UZFG-Y - RVO:67985904 ; BFU-R - RVO:68081707 ; UOCHB-X - RVO:61388963 UT WOS 000437038400010 EID SCOPUS 85049308381 DOI 10.1016/j.biomaterials.2018.05.030 Anotace The blocking of specific protein-protein interactions using nanoparticles is an emerging alternative to small molecule-based therapeutic interventions. However, the nanoparticles designed as „artificial proteins“ generally require modification of their surface with (bio)organic molecules and/or polymers to ensure their selectivity and specificity of action. Here, we show that nanosized diamond crystals (nanodiamonds, NDs) without any synthetically installed (bio)organic interface enable the specific and efficient targeting of the family of extracellular signalling molecules known as fibroblast growth factors (FGFs). We found that low nanomolar solutions of detonation NDs with positive-potential strongly associate with multiple FGF ligands present at sub-nanomolar concentrations and effectively neutralize the effects of FGF signalling in cells without interfering with other growth factor systems and serum proteins unrelated to FGFs. We identified an evolutionarily conserved FGF recognition motif, 17 amino acids long, that contributes to the selectivity of the ND-FGF interaction. In addition, we inserted this motif into a de novo constructed chimeric protein, which significantly improved its interaction with NDs. We demonstrated that the interaction of NDs, as purely inorganic nanoparticles, with proteins can mitigate pathological FGF signalling and promote the restoration of cartilage growth in a mouse limb explant model. Based on our observations, we foresee that NDs may potentially be applied as nano therapeutics to neutralize disease-related activities of FGFs in vivo. Pracoviště Ústav živočišné fyziologie a genetiky Kontakt Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Rok sběru 2019
Počet záznamů: 1