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Molecular Mechanisms of Resistance against PSII-Inhibiting Herbicides in iAmaranthus retroflexus/i from the Czech Republic
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SYSNO ASEP 0588253 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Molecular Mechanisms of Resistance against PSII-Inhibiting Herbicides in iAmaranthus retroflexus/i from the Czech Republic Tvůrce(i) Mikulka, J. (CZ)
Sen, M. K. (CZ)
Košnarová, P. (CZ)
Hamouz, P. (CZ)
Hamouzová, K. (CZ)
Sur, Vishma Pratap (MBU-M)
Suk, J. (CZ)
Bhattacharya, S. (CZ)
Soukup, J. (CZ)Číslo článku 904 Zdroj.dok. Genes. - : MDPI - ISSN 2073-4425
Roč. 15, č. 7 (2024)Poč.str. 13 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova chlorophyll fluorescence ; d1 protein ; cross-resistance ; supersensitivity ; identification ; metribuzin ; docking ; thr ; D1 protein mutations ; molecular docking ; chlorophyll fluorescence Obor OECD Genetics and heredity (medical genetics to be 3) Způsob publikování Omezený přístup Institucionální podpora MBU-M - RVO:61388971 UT WOS 001278383500001 EID SCOPUS 85199920391 DOI https://doi.org/10.3390/genes15070904 Anotace Amaranthus retroflexus L. (redroot pigweed) is one of the most problematic weeds in maize, sugar beet, vegetables, and soybean crop fields in Europe. Two pigweed amaranth biotypes (R1 and R2) from the Czech Republic resistant to photosystem II (PSII)-inhibiting herbicides were analyzed in this study. This study aimed to identify the genetic mechanisms that underlie the resistance observed in the biotypes. Additionally, we also intended to establish the use of chlorophyll fluorescence measurement as a rapid and reliable method for confirming herbicide resistance in this weed species. Both biotypes analyzed showed high resistance factors in a dose-response study and were thus confirmed to be resistant to PSII-inhibiting herbicides. A sequence analysis of the D1 protein revealed a well-known Ser-Gly substitution at amino acid position 264 in both biotypes. Molecular docking studies, along with the wild-type and mutant D1 protein's secondary structure analyses, revealed that the S264G mutation did not reduce herbicide affinity but instead indirectly affected the interaction between the target protein and the herbicides. The current study identified the S264G mutation as being responsible for conferring herbicide resistance in the pigweed amaranth biotypes. These findings can provide a strong basis for future studies that might use protein structure and mutation-based approaches to gain further insights into the detailed mechanisms of resistance in this weed species. In many individuals from both biotypes, resistance at a very early stage (BBCH10) of plants was demonstrated several hours after the application of the active ingredients by the chlorophyll fluorescence method. The effective PS II quantum yield parameter can be used as a rapid diagnostic tool for distinguishing between sensitive and resistant plants on an individual level. This method can be useful for identifying herbicide-resistant weed biotypes in the field, which can help farmers and weed management practitioners develop more effective weed control tactics. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2025 Elektronická adresa https://www.mdpi.com/2073-4425/15/7/904
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