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Potential of Electrospun Fibrous Scaffolds for Intestinal, Skin, and Lung Epithelial Tissue Modeling
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SYSNO ASEP 0580711 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Potential of Electrospun Fibrous Scaffolds for Intestinal, Skin, and Lung Epithelial Tissue Modeling Tvůrce(i) Goncalves, A.M. (PT)
Leal, F. (PT)
Moreira, A. (PT)
Schellhorn, T. (AT)
Hefka Blahnová, Veronika (UEM-P)
Zeiringer, S. (AT)
Vocetková, Karolína (UEM-P) ORCID
Tetyczka, C. (AT)
Simaite, A. (CZ)
Buzgo, M. (PT)
Roblegg, E. (AT)
Costa, P.F.F. (PT)
Ertl, P. (AT)
Filová, Eva (UEM-P) RID, ORCID
Kohl, Y. (DE)Zdroj.dok. Advanced NanoBiomed Research. - : Wiley-VCH Verlag GmbH - ISSN 2699-9307
Roč. 3, č. 4 (2023)Poč.str. 19 s. Jazyk dok. eng - angličtina Země vyd. DE - Německo Klíč. slova 3R principle ; cellulose ; electrospinning ; epithelial barrier ; in vitro modelst ; toxicological screening Obor OECD Biomaterials (as related to medical implants, devices, sensors) CEP FV40437 GA MPO - Ministerstvo průmyslu a obchodu Způsob publikování Open access Institucionální podpora UEM-P - RVO:68378041 UT WOS 000921305800001 EID SCOPUS 85165507957 DOI https://doi.org/10.1002/anbr.202200104 Anotace
Herein, intestinal, skin, and pulmonary in vitro tissue models based on electrospun membranes of poly(epsilon-caprolactone) (PCL) and cellulose acetate (CA), cellulose acetate phthalate (CAP), ethylcellulose (EC), or methylcellulose (MC) are presented. Physicochemical characterization and biocompatibility analyses of the scaffolds are carried out using colorectal adenocarcinoma cells (intestine), keratinocytes and fibroblasts (skin), and bronchial and alveolar epithelial cells (lung). PCL, PCL:CA, and PCL:EC are composed of nanofibers, whereas PCL:CAP and PCL:MC scaffolds comprise a combination of micro- and nanofibers. PCL, PCL:CA, PCL:CAP, and PCL:EC samples demonstrate water contact angles greater than 90 degrees and are, therefore, hydrophobic, while PCL:MC mats display a hydrophilic behavior. In intestinal models, cells adhere and proliferate on all scaffolds, in turn, studies with skin cell models reveal that PCL:CA and PCL:CAP blends outperform all other substrates. Lung cell models show that, while 16HBE cells adhere to and proliferate in PCL, PCL:CA, PCL:EC, and PCL:MC scaffolds, A549 cells only have the same biological response on PCL, PCL:CA, and PCL:MC. In summary, all fibrous meshes prepared are biocompatible toward most cell types tested, thus suggesting the potential of PCL-cellulose derivative blends as substrates suitable for in vitro epithelial tissue modeling and toxicity screening.Pracoviště Ústav experimentální medicíny Kontakt Arzuv Čaryjeva, arzuv.caryjeva@iem.cas.cz, Tel.: 241 062 218, 296 442 218 Rok sběru 2024 Elektronická adresa https://onlinelibrary.wiley.com/doi/10.1002/anbr.202200104
Počet záznamů: 1