Počet záznamů: 1
Rht18 Semidwarfism in Wheat Is Due to Increased GA 2-oxidaseA9 Expression and Reduced GA Content
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SYSNO ASEP 0489461 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Rht18 Semidwarfism in Wheat Is Due to Increased GA 2-oxidaseA9 Expression and Reduced GA Content Tvůrce(i) Ford, B. A. (AU)
Foo, E. (AU)
Sharwood, R. (AU)
Karafiátová, Miroslava (UEB-Q) RID, ORCID
Vrána, Jan (UEB-Q) RID, ORCID
MacMillan, C. (AU)
Nichols, D. S. (AU)
Steuernagel, B. (GB)
Uauy, C. (GB)
Doležel, Jaroslav (UEB-Q) RID, ORCID
Chandler, P. M. (GB)
Spielmeyer, W. (AU)Celkový počet autorů 12 Zdroj.dok. Plant Physiology. - : Oxford University Press - ISSN 0032-0889
Roč. 177, č. 1 (2018), s. 168-180Poč.str. 13 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova green-revolution ; gibberellin biosynthesis ; ectopic expression ; common wheat ; gene ; rice ; barley ; mutant ; chromosomes Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Genetics and heredity (medical genetics to be 3) CEP LO1204 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy GBP501/12/G090 GA ČR - Grantová agentura ČR Institucionální podpora UEB-Q - RVO:61389030 UT WOS 000431347500017 DOI Anotace Semidwarfing genes have improved crop yield by reducing height, improving lodging resistance, and allowing plants to allocate more assimilates to grain growth. In wheat (Triticum aestivum), the Rht18 semidwarfing gene was identified and deployed in durum wheat before it was transferred into bread wheat, where it was shown to have agronomic potential. Rht18, a dominant and gibberellin (GA) responsive mutant, is genetically and functionally distinct from the widely used GA-insensitive semidwarfing genes Rht-B1b and Rht-D1b. In this study, the Rht18 gene was identified by mutagenizing the semidwarf durum cultivar Icaro (Rht18) and generating mutants with a range of tall phenotypes. Isolating and sequencing chromosome 6A of these overgrowth mutants showed that they contained independent mutations in the coding region of GA2oxA9. GA2oxA9 is predicted to encode a GA 2-oxidase that metabolizes GA biosynthetic intermediates into inactive products, effectively reducing the amount of bioactive GA (GA1). Functional analysis of the GA2oxA9 protein demonstrated that GA2oxA9 converts the intermediate GA12 to the inactive metabolite GA110. Furthermore, Rht18 showed higher expression of GA2oxA9 and lower GA content compared with its tall parent. These data indicate that the increased expression of GA2oxA9 in Rht18 results in a reduction of both bioactive GA content and plant height. This study describes a height-reducing mechanism that can generate new genetic diversity for semidwarfism in wheat by combining increased expression with mutations of specific amino acid residues in GA2oxA9. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2019
Počet záznamů: 1