Počet záznamů: 1  

Rht18 Semidwarfism in Wheat Is Due to Increased GA 2-oxidaseA9 Expression and Reduced GA Content

  1. 1.
    SYSNO ASEP0489461
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    Poddruh JČlánek ve WOS
    NázevRht18 Semidwarfism in Wheat Is Due to Increased GA 2-oxidaseA9 Expression and Reduced GA Content
    Tvůrce(i) Ford, B. A. (AU)
    Foo, E. (AU)
    Sharwood, R. (AU)
    Karafiátová, Miroslava (UEB-Q) RID, ORCID
    Vrána, Jan (UEB-Q) RID, ORCID
    MacMillan, C. (AU)
    Nichols, D. S. (AU)
    Steuernagel, B. (GB)
    Uauy, C. (GB)
    Doležel, Jaroslav (UEB-Q) RID, ORCID
    Chandler, P. M. (GB)
    Spielmeyer, W. (AU)
    Celkový počet autorů12
    Zdroj.dok.Plant Physiology. - : Oxford University Press - ISSN 0032-0889
    Roč. 177, č. 1 (2018), s. 168-180
    Poč.str.13 s.
    Jazyk dok.eng - angličtina
    Země vyd.US - Spojené státy americké
    Klíč. slovagreen-revolution ; gibberellin biosynthesis ; ectopic expression ; common wheat ; gene ; rice ; barley ; mutant ; chromosomes
    Vědní obor RIVEB - Genetika a molekulární biologie
    Obor OECDGenetics and heredity (medical genetics to be 3)
    CEPLO1204 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
    GBP501/12/G090 GA ČR - Grantová agentura ČR
    Institucionální podporaUEB-Q - RVO:61389030
    UT WOS000431347500017
    DOI10.1104/pp.18.00023
    AnotaceSemidwarfing genes have improved crop yield by reducing height, improving lodging resistance, and allowing plants to allocate more assimilates to grain growth. In wheat (Triticum aestivum), the Rht18 semidwarfing gene was identified and deployed in durum wheat before it was transferred into bread wheat, where it was shown to have agronomic potential. Rht18, a dominant and gibberellin (GA) responsive mutant, is genetically and functionally distinct from the widely used GA-insensitive semidwarfing genes Rht-B1b and Rht-D1b. In this study, the Rht18 gene was identified by mutagenizing the semidwarf durum cultivar Icaro (Rht18) and generating mutants with a range of tall phenotypes. Isolating and sequencing chromosome 6A of these overgrowth mutants showed that they contained independent mutations in the coding region of GA2oxA9. GA2oxA9 is predicted to encode a GA 2-oxidase that metabolizes GA biosynthetic intermediates into inactive products, effectively reducing the amount of bioactive GA (GA1). Functional analysis of the GA2oxA9 protein demonstrated that GA2oxA9 converts the intermediate GA12 to the inactive metabolite GA110. Furthermore, Rht18 showed higher expression of GA2oxA9 and lower GA content compared with its tall parent. These data indicate that the increased expression of GA2oxA9 in Rht18 results in a reduction of both bioactive GA content and plant height. This study describes a height-reducing mechanism that can generate new genetic diversity for semidwarfism in wheat by combining increased expression with mutations of specific amino acid residues in GA2oxA9.
    PracovištěÚstav experimentální botaniky
    KontaktDavid Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
    Rok sběru2019
Počet záznamů: 1  

  Tyto stránky využívají soubory cookies, které usnadňují jejich prohlížení. Další informace o tom jak používáme cookies.