Počet záznamů: 1
Features of the organization of bread wheat chromosome 5BS based on physical mapping
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SYSNO ASEP 0488796 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Features of the organization of bread wheat chromosome 5BS based on physical mapping Tvůrce(i) Salina, E.A. (CU)
Nesterov, V. (RU)
Frenkel, Z. (IL)
Kiseleva, V. I. (RU)
Timonova, E. M. (RU)
Magni, F. (IT)
Vrána, Jan (UEB-Q) RID, ORCID
Šafář, Jan (UEB-Q) RID, ORCID
Šimková, Hana (UEB-Q) RID, ORCID
Doležel, Jaroslav (UEB-Q) RID, ORCID
Korol, A. (IL)
Sergeeva, E.M. (RU)Celkový počet autorů 12 Číslo článku 80 Zdroj.dok. BMC Genomics. - : BioMed Central - ISSN 1471-2164
Roč. 19, FEB 9 (2018)Poč.str. 13 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova Chromosome 5BS ; Genetic markers ; Hexaploid wheat ; Physical mapping ; Sequencing ; Synteny ; Triticum aestivum Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Genetics and heredity (medical genetics to be 3) CEP GBP501/12/G090 GA ČR - Grantová agentura ČR LO1204 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora UEB-Q - RVO:61389030 UT WOS 000424782900011 EID SCOPUS 85041808764 DOI 10.1186/s12864-018-4470-y Anotace Background: The IWGSC strategy for construction of the reference sequence of the bread wheat genome is based on first obtaining physical maps of the individual chromosomes. Our aim is to develop and use the physical map for analysis of the organization of the short arm of wheat chromosome 5B (5BS) which bears a number of agronomically important genes, including genes conferring resistance to fungal diseases. Results: A physical map of the 5BS arm (290 Mbp) was constructed using restriction fingerprinting and LTC software for contig assembly of 43,776 BAC clones. The resulting physical map covered ~ 99% of the 5BS chromosome arm (111 scaffolds, N50 = 3.078 Mb). SSR, ISBP and zipper markers were employed for anchoring the BAC clones, and from these 722 novel markers were developed based on previously obtained data from partial sequencing of 5BS. The markers were mapped using a set of Chinese Spring (CS) deletion lines, and F2 and RICL populations from a cross of CS and CS-5B dicoccoides. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2019
Počet záznamů: 1