Počet záznamů: 1
Evolutionary conserved cysteines function as cis-acting regulators of arabidopsis PIN-FORMED 2 distribution
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SYSNO ASEP 0485766 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Evolutionary conserved cysteines function as cis-acting regulators of arabidopsis PIN-FORMED 2 distribution Tvůrce(i) Retzer, Katarzyna (UEB-Q) ORCID
Lacek, Jozef (UEB-Q) ORCID
Skokan, Roman (UEB-Q) ORCID
Del Genio, C. H. (GB)
Vosolsobě, S. (CZ)
Laňková, Martina (UEB-Q) RID, ORCID
Malínská, Kateřina (UEB-Q) RID, ORCID
Konstantinova, N. (AT)
Zažímalová, Eva (UEB-Q) RID, ORCID
Napier, R. M. (GB)
Petrášek, Jan (UEB-Q) RID, ORCID
Luschnig, C. (AT)Celkový počet autorů 12 Číslo článku 2274 Zdroj.dok. International Journal of Molecular Sciences. - : MDPI
Roč. 18, č. 11 (2017)Poč.str. 20 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova Arabidopsis ; Auxin ; Intracellular distribution ; PIN proteins ; Plasma membrane protein sorting ; Protein mobility ; Protein modeling ; Root phenotype ; srrf Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Plant sciences, botany CEP GAP305/11/0797 GA ČR - Grantová agentura ČR Institucionální podpora UEB-Q - RVO:61389030 UT WOS 000416811300048 EID SCOPUS 85032666379 DOI 10.3390/ijms18112274 Anotace Coordination of plant development requires modulation of growth responses that are under control of the phytohormone auxin. PIN-FORMED plasma membrane proteins, involved in intercellular transport of the growth regulator, are key to the transmission of such auxin signals and subject to multilevel surveillance mechanisms, including reversible post-translational modifications. Apart from well-studied PIN protein modifications, namely phosphorylation and ubiquitylation, no further post-translational modifications have been described so far. Here, we focused on root-specific Arabidopsis PIN2 and explored functional implications of two evolutionary conserved cysteines, by a combination of in silico and molecular approaches. PIN2 sequence alignments and modeling predictions indicated that both cysteines are facing the cytoplasm and therefore would be accessible to redox status-controlled modifications. Notably, mutant pin2 C−A alleles retained functionality, demonstrated by their ability to almost completely rescue defects of a pin2 null allele, whereas high resolution analysis of pin2 C−A localization revealed increased intracellular accumulation, and altered protein distribution within plasma membrane micro-domains. The observed effects of cysteine replacements on root growth and PIN2 localization are consistent with a model in which redox status-dependent cysteine modifications participate in the regulation of PIN2 mobility, thereby fine-tuning polar auxin transport. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2018
Počet záznamů: 1