Počet záznamů: 1
Sequencing flow-sorted short arm of Haynaldia villosa chromosome 4V provides insights into its molecular structure and virtual gene order
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SYSNO ASEP 0481223 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Sequencing flow-sorted short arm of Haynaldia villosa chromosome 4V provides insights into its molecular structure and virtual gene order Tvůrce(i) Xiao, J. (CN)
Dai, K. (CN)
Fu, S. (CN)
Vrána, Jan (UEB-Q) RID, ORCID
Kubaláková, Marie (UEB-Q) RID
Wan, W. (CN)
Sun, H. (CN)
Zhao, J. (CN)
Yu, C. (GB)
Wu, Y. (CN)
Abrouk, Michael (UEB-Q) RID
Wang, H. (CN)
Doležel, Jaroslav (UEB-Q) RID, ORCID
Wang, X. (CN)Celkový počet autorů 14 Číslo článku 791 Zdroj.dok. BMC Genomics. - : BioMed Central - ISSN 1471-2164
Roč. 18, č. 1 (2017)Poč.str. 11 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova Chromosome arm 4VS ; Flow sorting ; Genome zipper ; Haynaldia villosa ; Scaffold Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Plant sciences, botany CEP LO1204 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy GBP501/12/G090 GA ČR - Grantová agentura ČR Institucionální podpora UEB-Q - RVO:61389030 UT WOS 000413466800006 EID SCOPUS 85031501820 DOI 10.1186/s12864-017-4211-7 Anotace In this work, the short arm of H. villosa chromosome 4V (4VS) was sorted by flow cytometry and sequenced using Illumina platform. About 170.6 Mb assembled sequences were obtained. Further analysis showed that repetitive elements accounted for about 64.6% of 4VS, while the coding fraction, which is corresponding to 1977 annotated genes, represented 1.5% of the arm. The syntenic regions of the 4VS were searched and identified on wheat group 4 chromosomes 4AL, 4BS, 4DS, Brachypodium chromosomes 1 and 4, rice chromosomes 3 and 11, and sorghum chromosomes 1, 5 and 8. Based on genome-zipper analysis, a virtual gene order comprising 735 gene loci on 4VS genome was built by referring to the Brachypodium genome, which was relatively consistent with the scaffold order determined for Ae. tauschii chromosome 4D. The homologous alleles of several cloned genes on wheat group 4 chromosomes including Rht-1 gene were identified. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2018
Počet záznamů: 1