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Histological and biochemical response of Norway spruce somatic embryos to UV-B irradiation
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SYSNO ASEP 0476855 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Histological and biochemical response of Norway spruce somatic embryos to UV-B irradiation Tvůrce(i) Eliášová, Kateřina (UEB-Q) RID, ORCID
Vondráková, Zuzana (UEB-Q) RID, ORCID
Malbeck, Jiří (UEB-Q) RID, ORCID
Trávníčková, Alena (UEB-Q) ORCID
Pešek, Bedřich (UEB-Q) RID
Vágner, Martin (UEB-Q) RID, ORCID
Cvikrová, Milena (UEB-Q) RID, ORCIDCelkový počet autorů 7 Zdroj.dok. Trees: structure and function. - : Springer - ISSN 0931-1890
Roč. 31, č. 4 (2017), s. 1279-1293Poč.str. 15 s. Jazyk dok. eng - angličtina Země vyd. DE - Německo Klíč. slova Oxidative stress ; Phenolic acids ; Phenylpropanoids ; Picea abies (L.) Karst ; Polyamines ; Somatic embryogenesis Vědní obor RIV ED - Fyziologie Obor OECD Forestry CEP LD13050 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy LD13051 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora UEB-Q - RVO:61389030 UT WOS 000405685800015 EID SCOPUS 85018831070 DOI 10.1007/s00468-017-1547-1 Anotace Plants respond to UV-B exposure by synthesizing a broad range of secondary metabolites, including ROS-scavenging antioxidants and UV-B screening phenylpropanoids. Our recent results indicated that the accumulation of higher levels of polyamines in fully developed somatic embryos of Norway spruce may be causally linked to better tolerance of UV-B irradiation. In the present work, we concentrated primarily on changes in phenolics and their localization in irradiated embryos. Somatic embryos, after 14 days of desiccation, were exposed to 6 W m(-2) h(-1) of 312 nm UV-B radiation. The accumulation of higher levels of spermidine and spermine (by about 20%) and total phenolics (about 25%) in these embryos indicates their participation in stress response. Histological localization of polyphenolic compounds was performed under a transmission light microscope. UV-B radiation elicited the accumulation of polyphenolics in the junction zone between root cap and hypocotyl and in the epidermal and subepidermal cells of hypocotyl and cotyledons. The deposition of polyphenolics in intact epidermal cells which were located in the neighbourhood of damaged cells may demonstrate that there is potential for transmission of a signal from injured cells to tissues protected against the direct effects of UV-B irradiation. UV-B irradiation evoked striking polyphenolic accumulation in specialized idioblastic cells localized beneath the epidermis of the somatic embryo hypocotyl and cotyledons. The fluorescence due to flavonoids, detected under confocal laser scanning microscope, increased dramatically after UV-B irradiation in the epidermis of the hypocotyl and cotyledons and in the surface of the root cap of spruce somatic embryos. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2018
Počet záznamů: 1