Construction of a map-based reference genome sequence for barley, Hordeum vulgare L.

0476491 - ÚEB 2018 RIV GB eng J - Článek v odborném periodiku
Beier, S. - Himmelbach, A. - Colmsee, C. - Zhang, X. Q. - Barrero, R. A. - Hastie, A. - Šimková, Hana - Staňková, Helena - Vrána, Jan - Chan, S. - Zhou, G. - Poland, J. - Bellgard, M. I. - Houben, A. - Doležel, Jaroslav - Ayling, S. - Lonardi, S. - Scholz, U. - Stein, N. - Mascher, M. … celkem 60 autorů
Construction of a map-based reference genome sequence for barley, Hordeum vulgare L.
Scientific Data. Roč. 4, APR 27 (2017), č. článku 170044. E-ISSN 2052-4463
Grant CEP: GA MŠMT(CZ) LO1204
Institucionální podpora: RVO:61389030
Klíčová slova: BACTERIAL ARTIFICIAL CHROMOSOMES * PHYSICAL MAP * LIBRARIES
Obor OECD: Plant sciences, botany
Impakt faktor: 5.311, rok: 2017

Barley (Hordeum vulgare L.) is a cereal grass mainly used as animal fodder and raw material for the malting industry. The map-based reference genome sequence of barley cv. a Morex was constructed by the International Barley Genome Sequencing Consortium (IBSC) using hierarchical shotgun sequencing. Here, we report the experimental and computational procedures to (i) sequence and assemble more than 80,000 bacterial artificial chromosome (BAC) clones along the minimum tiling path of a genome-wide physical map, (ii) find and validate overlaps between adjacent BACs, (iii) construct 4,265 non-redundant sequence scaffolds representing clusters of overlapping BACs, and (iv) order and orient these BAC clusters along the seven barley chromosomes using positional information provided by dense genetic maps, an optical map and chromosome conformation capture sequencing (Hi-C). Integrative access to these sequence and mapping resources is provided by the barley genome explorer (BARLEX).
Trvalý link: http://hdl.handle.net/11104/0272979