Počet záznamů: 1
Microscale magnetic microparticle-based immunopurification of cytokinins from Arabidopsis root apex
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SYSNO ASEP 0476413 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Microscale magnetic microparticle-based immunopurification of cytokinins from Arabidopsis root apex Tvůrce(i) Plačková, Lenka (UEB-Q) ORCID, RID
Oklešťková, Jana (UEB-Q) RID, ORCID, SAI
Pospíšková, K. (CZ)
Poláková, K. (CZ)
Buček, J. (CZ)
Stýskala, J. (CZ)
Zatloukal, M. (CZ)
Šafařík, Ivo (BC-A) RID, ORCID
Zbořil, R. (CZ)
Strnad, Miroslav (UEB-Q) RID, ORCID
Doležal, Karel (UEB-Q) RID, ORCID
Novák, Ondřej (UEB-Q) RID, ORCID, SAICelkový počet autorů 12 Zdroj.dok. Plant Journal. - : Wiley - ISSN 0960-7412
Roč. 89, č. 5 (2017), s. 1065-1075Poč.str. 11 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova solid-phase extraction ; tandem mass-spectrometry ; endogenous cytokinins ; plant-samples ; cis-zeatin ; quantitative-analysis ; aromatic cytokinins ; abscisic-acid ; oryza-sativa ; purification ; cytokinins ; magnetic microparticles ; immunoaffinity separation ; metabolite profiling ; Arabidopsis thaliana ; technical advance Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Plant sciences, botany CEP LO1204 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy LK21306 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy GA14-34792S GA ČR - Grantová agentura ČR Institucionální podpora UEB-Q - RVO:61389030 ; BC-A - RVO:60077344 UT WOS 000395810800017 EID SCOPUS 85013471725 DOI https://doi.org/10.1111/tpj.13443 Anotace Cytokinins (CKs) are pivotal plant hormones that have crucial roles in plant growth and development. However, their isolation and quantification are usually challenging because of their extremely low levels in plant tissues (pmol g(-1) fresh weight). We have developed a simple microscale magnetic immunoaffinity-based method for selective one-step isolation of CKs from very small amounts of plant tissue (less than 0.1 mg fresh weight). The capacity of the immunosorbent and the effect of the complex plant matrix on the yield of the rapid one-step purification were tested using a wide range of CK concentrations. The total recovery range of the new microscale isolation procedure was found to be 30-80% depending on individual CKs. Immunoaffinity extraction using group-specific monoclonal CK antibodies immobilized onto magnetic microparticles was combined with a highly sensitive ultrafast mass spectrometry-based method with a detection limit close to one attomole. This combined approach allowed metabolic profiling of a wide range of naturally occurring CKs (bases, ribosides and N-9-glucosides) in 1.0-mm sections of the Arabidopsis thaliana root meristematic zone. The magnetic immunoaffinity separation method was shown to be a simple and extremely fast procedure requiring minimal amounts of plant tissue. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2018
Počet záznamů: 1