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A High Resolution Radiation Hybrid Map of Wheat Chromosome 4A
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SYSNO ASEP 0473676 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název A High Resolution Radiation Hybrid Map of Wheat Chromosome 4A Tvůrce(i) Balcárková, Barbora (UEB-Q)
Frenkel, Z. (IL)
Škopová, Monika (UEB-Q)
Abrouk, Michael (UEB-Q) RID
Kumar, A. (US)
Chao, S. (US)
Kianian, S. F. (US)
Akhunov, E. (US)
Korol, A. (IL)
Doležel, Jaroslav (UEB-Q) RID, ORCID
Valárik, Miroslav (UEB-Q) RID, ORCIDCelkový počet autorů 11 Číslo článku 2063 Zdroj.dok. Frontiers in Plant Science. - : Frontiers Research Foundation - ISSN 1664-462X
Roč. 7, JAN 10 (2017)Poč.str. 14 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova triticum-aestivum l. ; bread wheat ; high-density ; agronomic traits ; tetraploid wheat ; hexaploid wheat ; polyploid wheat ; genetic maps ; genomes ; recombination ; endosperm radiation hybrid panel ; radiation hybrid map ; wheat chromosome 4A ; chromosome deletion bin map ; Triticum aestivum ; SNP iSelect array Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Plant sciences, botany CEP LO1204 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy GA14-07164S GA ČR - Grantová agentura ČR Institucionální podpora UEB-Q - RVO:61389030 UT WOS 000391502500001 DOI 10.3389/fpls.2016.02063 Anotace Bread wheat has a large and complex allohexaploid genome with low recombination level at chromosome centromeric and peri-centromeric regions. This significantly hampers ordering of markers, contigs of physical maps and sequence scaffolds and impedes obtaining of high-quality reference genome sequence. Here we report on the construction of high-density and high-resolution radiation hybrid (RH) map of chromosome 4A supported by high-density chromosome deletion map. A total of 119 endosperm-based RH lines of two RH panels and 15 chromosome deletion bin lines were genotyped with 90K iSelect single nucleotide polymorphism (SNP) array. A total of 2316 and 2695 markers were successfully mapped to the 4A RH and deletion maps, respectively. The chromosome deletion map was ordered in 19 bins and allowed precise identification of centromeric region and verification of the RH panel reliability. The 4A-specific RH map comprises 1080 mapping bins and spans 6550.9 cR with a resolution of 0.13 Mb/cR. Significantly higher mapping resolution in the centromeric region was observed as compared to recombination maps. Relatively even distribution of deletion frequency along the chromosome in the RH panel was observed and putative functional centromere was delimited within a region characterized by two SNP markers. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2018
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