Počet záznamů: 1
Protein as a sole source of nitrogen for in vitro grown tobacco plantlets
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SYSNO ASEP 0471229 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Protein as a sole source of nitrogen for in vitro grown tobacco plantlets Tvůrce(i) Synková, Helena (UEB-Q) RID, ORCID
Hýsková, V. (CZ)
Garčeková, K. (CZ)
Křížová, S. (CZ)
Ryšlavá, H. (CZ)Celkový počet autorů 5 Zdroj.dok. Biologia Plantarum. - : Ústav experimentální botaniky AV ČR, v. v. i. - ISSN 0006-3134
Roč. 60, č. 4 (2016), s. 635-644Poč.str. 10 s. Jazyk dok. eng - angličtina Země vyd. CZ - Česká republika Klíč. slova glutamate-dehydrogenase ; inorganic nitrogen ; nitrate transport ; organic nitrogen ; amino-acids ; metabolism ; seedlings ; leaves ; roots ; assimilation ; ammonium ; casein ; chlorophyll fluorescence ; nitrate ; photosynthesis Vědní obor RIV EF - Botanika Institucionální podpora UEB-Q - RVO:61389030 UT WOS 000392128700004 DOI https://doi.org/10.1007/s10535-016-0639-x Anotace We tested the capability of plants to utilize protein as the exclusive source of nitrogen. The aim of this study was to find out how such a nutrition affected plantlet growth, photosynthetic performance, and N assimilation metabolism in tobacco (Nicotiana tabacum L., cv. Petit Havana SR1) grown in vitro. Plantlets grown in a casein-supplemented (CA) medium were compared to plantlets grown in a complete Murashige-Skoog (MS) medium, plantlets grown in an ammonium-deficient medium (N1), or plantlets grown in a nitrate-reduced medium (N2). In addition, the plantlets were grown in the presence or absence of 1.5 % (m/v) saccharose as an additional carbon source. Casein, similarly as inorganic N limitation, reduced generally plantlet growth, whereas no significant effects were observed on photosynthetic parameters evaluated by chlorophyll a fluorescence. Although addition of saccharose stimulated the plantlet growth particularly in the MS, it showed a rather negative influence both on the growth and on the photochemical efficiency of photosystem II in the plantlets grown in the CA and N1. The activities of enzymes involved in N assimilation, such as nitrate reductase (NR) and glutamine synthetase (GS), were lower in the plantlets grown in the CA, N1, and N2, both in leaves and in roots. On the other hand, glutamate synthase and glutamate dehydrogenase were employed by the plantlets grown in the CA. The presence of saccharose in the growth medium stimulated mainly NR and GS activities in the MS grown plantlets, whereas enzyme activities of the plantlets grown on the N1, N2, and CA were not significantly influenced. We proved that the tobacco plantlets can utilize casein as the sole source of N particularly during their photoautotrophic cultivation. Contrary to positive effects of photomixotrophic nutrition for the MS grown plantlets, exogenous sugar seemed to diminish the ability of the casein-supplemented plantlets to utilize efficiently the additional C source. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2017
Počet záznamů: 1