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In Vitro and In Vivo Comparison of Selected Ga-68 and Zr-89 Labelled Siderophores
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SYSNO ASEP 0461642 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název In Vitro and In Vivo Comparison of Selected Ga-68 and Zr-89 Labelled Siderophores Tvůrce(i) Petřík, M. (CZ)
Zhai, C. (AT)
Nový, Z. (CZ)
Urbánek, Lubor (UEB-Q) RID
Haas, H. (AT)
Decristoforo, C. (AT)Zdroj.dok. Molecular Imaging and Biology. - : Springer - ISSN 1536-1632
Roč. 18, č. 3 (2016), s. 344-352Poč.str. 9 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova Siderophores ; Gallium-68 ; Zirconium-89 Vědní obor RIV EB - Genetika a molekulární biologie CEP LO1304 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy LO1204 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy TE01020028 GA TA ČR - Technologická agentura ČR Institucionální podpora UEB-Q - RVO:61389030 UT WOS 000376623600005 DOI 10.1007/s11307-015-0897-6 Anotace Some [Ga-68]siderophores show promise in specific and sensitive imaging of infection. Here, we compare the in vitro and in vivo behaviour of selected Ga-68 and Zr-89 labelled siderophores.
Radiolabelling was performed in HEPES or sodium acetate buffer systems. Radiochemical purity of labelled siderophores was determined using chromatography. Partition coefficients, in vitro stability and protein binding affinities were determined. Ex vivo biodistribution and animal imaging was studied in mice.
Certain differences among studied siderophores were observed in labelling efficiency. Protein binding and stability tests showed highest stabilities and lowest protein binding affinities for Ga-68 and [Zr-89]triacetylfusarinine C (TAFC). All studied Ga-68 and [Zr-89]siderophores exhibited a similar biodistribution and pharmacokinetics in mice with the exception of [Zr-89]ferrioxamine E (FOXE).
Zr-89 and [Ga-68]siderophores showed analogous in vitro and in vivo behaviour. Tested [Zr-89]siderophores could be applied for longitudinal positron emission tomography (PET) studies of fungal infections and especially TAFC for the development of novel bioconjugates.Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2017
Počet záznamů: 1