Počet záznamů: 1
Arabidopsis FH1 Formin Affects Cotyledon Pavement Cell Shape by Modulating Cytoskeleton Dynamics
- 1.
SYSNO ASEP 0461618 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Arabidopsis FH1 Formin Affects Cotyledon Pavement Cell Shape by Modulating Cytoskeleton Dynamics Tvůrce(i) Rosero, A. (CZ)
Oulehlová, Denisa (UEB-Q) RID
Stillerová, L. (CZ)
Schiebertová, P. (CZ)
Gunt, M. (CZ)
Žárský, Viktor (UEB-Q) RID, ORCID
Cvrčková, F. (CZ)Zdroj.dok. Plant and Cell Physiology. - : Oxford University Press - ISSN 0032-0781
Roč. 57, č. 3 (2016), s. 488-504Poč.str. 17 s. Jazyk dok. eng - angličtina Země vyd. JP - Japonsko Klíč. slova Arabidopsis thaliana ; Confocal microscopy ; Cotyledon pavement cells Vědní obor RIV ED - Fyziologie Institucionální podpora UEB-Q - RVO:61389030 UT WOS 000374572400005 DOI 10.1093/pcp/pcv209 Anotace Plant cell morphogenesis involves concerted rearrangements of microtubules and actin microfilaments. We previously reported that FH1, the main Arabidopsis thaliana housekeeping Class I membrane-anchored formin, contributes to actin dynamics and microtubule stability in rhizodermis cells. Here we examine the effects of mutations affecting FH1 (At3g25500) on cell morphogenesis and above-ground organ development in seedlings, as well as on cytoskeletal organization and dynamics, using a combination of confocal and variable angle epifluorescence microscopy with a pharmacological approach. Homozygous fh1 mutants exhibited cotyledon epinasty and had larger cotyledon pavement cells with more pronounced lobes than the wild type. The pavement cell shape alterations were enhanced by expression of the fluorescent microtubule marker GFP-microtubule-associated protein 4 (MAP4). Mutant cotyledon pavement cells exhibited reduced density and increased stability of microfilament bundles, as well as enhanced dynamics of microtubules. Analogous results were also obtained upon treatments with the formin inhibitor SMIFH2 (small molecule inhibitor of formin homology 2 domains). Pavement cell shape in wild-type (wt) and fh1 plants in some situations exhibited a differential response towards anti-cytoskeletal drugs, especially the microtubule disruptor oryzalin. Our observations indicate that FH1 participates in the control of microtubule dynamics, possibly via its effects on actin, subsequently influencing cell morphogenesis and macroscopic organ development. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2017
Počet záznamů: 1