Počet záznamů: 1
Application of Concanavalin A during immune responsiveness skin-swelling tests facilitates measurement interpretation in mammalian ecology
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SYSNO ASEP 0461399 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Application of Concanavalin A during immune responsiveness skin-swelling tests facilitates measurement interpretation in mammalian ecology Tvůrce(i) Bílková, B. (CZ)
Albrecht, Tomáš (UBO-W) RID, SAI, ORCID
Chudíčková, Milada (UEM-P) RID, ORCID
Holáň, Vladimír (UEM-P) RID
Piálek, Jaroslav (UBO-W) RID, ORCID, SAI
Vinkler, M. (CZ)Celkový počet autorů 6 Zdroj.dok. Ecology and Evolution. - : Wiley - ISSN 2045-7758
Roč. 6, č. 13 (2016), s. 4551-4564Poč.str. 14 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova Concanavalin A ; cytokine ; ecoimmunology ; evolutionary immunology ; field immunoecological research ; histology ; leucocytes ; PHA-induced hypersensitivity ; phytohemagglutinin ; T-cell-mediated immunocompetence Vědní obor RIV EG - Zoologie CEP GA206/08/0640 GA ČR - Grantová agentura ČR GA15-11782S GA ČR - Grantová agentura ČR Institucionální podpora UBO-W - RVO:68081766 ; UEM-P - RVO:68378041 UT WOS 000379344400026 EID SCOPUS 84977516136 DOI 10.1002/ece3.2211 Anotace The skin-swelling test is a simple and widespread method used in field ecological research to estimate cellular immune responsiveness in animals. This immunoecological test is based on measuring the magnitude of tissue swelling response at specific times following subcutaneous application of an experimental pro-inflammatory stimulant. In the vast majority of studies across vertebrate taxa, phytohemagglutinin (PHA) is used as a universal stimulant. Given the complexity of immune response activation pathways of PHA, however, interpretation of test results can be ambiguous. The goal of this study was to improve methodology of the skin-swelling test. Based on previous evidence suggesting that mammalian T cells are readily activated by Concanavalin A (ConA) in vitro, we compared cellular immune responses in vivo to PHA and ConA as an alternative proinflammatory stimulant in mice. Our results corroborate that PHA and ConA show important differences in both dynamics and response amplitude in rodents. ConA induces stronger swelling with a distinct leukocyte activity pattern and higher proinflammatory cytokine and interferon gamma expression than PHA during peak response. We conclude that ConA is the more suitable stimulant for skin-swelling tests in mammals. Pracoviště Ústav biologie obratlovců Kontakt Hana Slabáková, slabakova@ivb.cz, Tel.: 543 422 524 Rok sběru 2017
Počet záznamů: 1