Počet záznamů: 1
Arabidopsis non-specific phospholipase C1: Characterization and its involvement in response to heat stress
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SYSNO ASEP 0451388 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Arabidopsis non-specific phospholipase C1: Characterization and its involvement in response to heat stress Tvůrce(i) Krčková, Zuzana (UEB-Q) ORCID
Brouzdová, Jitka (UEB-Q)
Daněk, Michal (UEB-Q) ORCID
Kocourková, Daniela (UEB-Q) ORCID
Rainteau, D. (FR)
Ruelland, E. (FR)
Valentová, O. (CZ)
Pejchar, Přemysl (UEB-Q) RID, ORCID
Martinec, Jan (UEB-Q) RID, ORCIDZdroj.dok. Frontiers in Plant Science. - : Frontiers Research Foundation - ISSN 1664-462X
Roč. 6, NOV 4 (2015), s. 928Poč.str. 13 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova Arabidopsis thaliana ; Diacylglycerol ; Heat stress Vědní obor RIV ED - Fyziologie CEP GAP501/12/1942 GA ČR - Grantová agentura ČR Institucionální podpora UEB-Q - RVO:61389030 UT WOS 000364581700001 EID SCOPUS 84947424867 DOI 10.3389/fpls.2015.00928 Anotace The Arabidopsis non-specific phospholipase C1 (NPC) protein family is encoded by the genes NPC1 – NPC6. It has been shown that NPC4 and NPC5 possess phospholipase C activity; NPC3 has lysophosphatidic acid phosphatase activity. NPC3, 4 and 5 play roles in the responses to hormones and abiotic stresses. NPC1, 2 and 6 has not been studied functionally yet. We found that Arabidopsis NPC1 expressed in Escherichia coli possesses phospholipase C activity in vitro. This protein was able to hydrolyse phosphatidylcholine to diacylglycerol. NPC1-green fluorescent protein was localized to secretory pathway compartments in Arabidopsis roots. In the knock out T-DNA insertion line NPC1 (npc1) basal thermotolerance was impaired compared with wild-type (WT); npc1 exhibited significant decreases in survival rate and chlorophyll content at the seventh day after heat stress (HS). Conversely, plants overexpressing NPC1 (NPC1-OE) were more resistant to HS compared with WT. These findings suggest that NPC1 is involved in the plant response to heat. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2016
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