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Whole-Cell Optical Biosensor for Mercury – Operational Conditions in Saline Water
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SYSNO ASEP 0443171 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Whole-Cell Optical Biosensor for Mercury – Operational Conditions in Saline Water Tvůrce(i) Solovyev, Andrey (UCHP-M) RID, ORCID, SAI
Kuncová, Gabriela (UCHP-M) RID, SAI
Demnerová, K. (CZ)Zdroj.dok. Chemical Papers. - : Springer - ISSN 0366-6352
Roč. 69, č. 1 (2015), s. 183-191Poč.str. 9 s. Jazyk dok. eng - angličtina Země vyd. PL - Polsko Klíč. slova mercury detection assay ; bioluminescent bioreporter ; sea water Vědní obor RIV CA - Anorganická chemie CEP TA03010544 GA TA ČR - Technologická agentura ČR Institucionální podpora UCHP-M - RVO:67985858 UT WOS 000350130100017 EID SCOPUS 84916597978 DOI 10.1515/chempap-2015-0009 Anotace The present study demonstrates the influences of chlorides, fluorides and bromides of potassium and sodium on the growth and Hg2+-induced bioluminescence of bioreporter Escherichia coli ARL1. In a Luria-Bertani medium,cell growth was inhibited by concentrations of sodium and potassium fluorides above 0.2 mol L-1. The addition of NaCl increased cell tolerance to the toxic effects of fluorides and bromides. Lag periods of 10 h and more were observed for cultivations in LB without NaCl and with halides at concentrations lower than 0.06 mol L-1. In a phosphate buffer (PB), the bioluminescence of E. coli ARL1, induced with HgCl2, was increased by the addition of NaCl, KCl, NaBr, KBr, NaF and KF (concentration of 0-0.25 mol L-1). In a saline phosphate buffer (PBS), the maxima of induced bioluminescence declined to 50%, in the case of NaF (0.12 mol L-1), and to zero for KF. An addition of tryptone to the induction medium increased induced light emission ten-fold. Concentrated artificial sea water (ASW) (70-100% ASW) inhibited bioluminescence induction. The new detection assay with E. coli ARL1 made possible the detection of 0.57 mu g L-1 of HgCl2 in double-diluted artificial sea water (25 % ASW). Pracoviště Ústav chemických procesů Kontakt Eva Jirsová, jirsova@icpf.cas.cz, Tel.: 220 390 227 Rok sběru 2016
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