Počet záznamů: 1
Bordetella Adenylate Cyclase Toxin Differentially Modulates Toll-Like Receptor-Stimulated Activation, Migration and T Cell Stimulatory Capacity of Dendritic Cells
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SYSNO ASEP 0435908 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Bordetella Adenylate Cyclase Toxin Differentially Modulates Toll-Like Receptor-Stimulated Activation, Migration and T Cell Stimulatory Capacity of Dendritic Cells Tvůrce(i) Adkins, Irena (MBU-M)
Kamanová, Jana (MBU-M) ORCID, RID
Kocourková, A. (CZ)
Švédová, Martina (MBU-M)
Tomala, Jakub (MBU-M) RID, ORCID
Janová, H. (CZ)
Mašín, Jiří (MBU-M) RID, ORCID
Chládková, Barbara (MBU-M) RID
Bumba, Ladislav (MBU-M) RID, ORCID
Kovář, Marek (MBU-M) RID, ORCID
Ross, P. J. (IE)
Tučková, Ludmila (MBU-M) RID
Spíšek, R. (CZ)
Mills, K. H. G. (IE)
Šebo, Peter (MBU-M) RID, ORCIDZdroj.dok. PLoS ONE. - : Public Library of Science - ISSN 1932-6203
Roč. 9, č. 8 (2014)Poč.str. 13 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova RESPIRATORY-INFECTION ; INTERLEUKIN-10 PRODUCTION ; PROTECTIVE IMMUNITY Vědní obor RIV EE - Mikrobiologie, virologie CEP GA310/08/0447 GA ČR - Grantová agentura ČR GP310/09/P582 GA ČR - Grantová agentura ČR GAP301/11/0325 GA ČR - Grantová agentura ČR 1M0506 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora MBU-M - RVO:61388971 UT WOS 000339819800123 DOI 10.1371/journal.pone.0104064 Anotace Adenylate cyclase toxin (CyaA) is a key virulence factor of the whooping cough agent Bordetella pertussis. The toxin targets CD11b-expressing phagocytes and delivers into their cytosol an adenylyl cyclase (AC) enzyme that subverts cellular signaling by increasing cAMP levels. In the present study, we analyzed the modulatory effects of CyaA on adhesive, migratory and antigen presenting properties of Toll-like receptor (TLR)-activated murine and human dendritic cells (DCs). cAMP signaling of CyaA enhanced TLR-induced dissolution of cell adhesive contacts and migration of DCs towards the lymph node-homing chemokines CCL19 and CCL21 in vitro. Moreover, we examined in detail the capacity of toxin-treated DCs to induce CD4(+) and CD8(+) T cell responses. Exposure to CyaA decreased the capacity of LPS-stimulated DCs to present soluble protein antigen to CD4(+) T cells independently of modulation of co-stimulatory molecules and cytokine production, and enhanced their capacity to promote CD4(+) CD25(+) Foxp3(+) T regulatory cells in vitro. In addition, CyaA decreased the capacity of LPS-stimulated DCs to induce CD8(+) T cell proliferation and limited the induction of IFN-gamma producing CD8(+) T cells while enhancing IL-10 and IL-17-production. These results indicate that through activation of cAMP signaling, the CyaA may be mobilizing DCs impaired in T cell stimulatory capacity and arrival of such DCs into draining lymph nodes may than contribute to delay and subversion of host immune responses during B. pertussis infection Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2015
Počet záznamů: 1