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Advances in plant chromosome genomics
- 1.
SYSNO ASEP 0429975 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Advances in plant chromosome genomics Tvůrce(i) Doležel, Jaroslav (UEB-Q) RID, ORCID
Vrána, Jan (UEB-Q) RID, ORCID
Cápal, Petr (UEB-Q) RID, ORCID
Kubaláková, Marie (UEB-Q) RID
Burešová, Veronika (UEB-Q) RID
Šimková, Hana (UEB-Q) RID, ORCIDZdroj.dok. Biotechnology Advances. - : Elsevier - ISSN 0734-9750
Roč. 32, č. 1 (2014), s. 122-136Poč.str. 15 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova BAC library ; Chromosome sorting ; Cytogenetics Vědní obor RIV EB - Genetika a molekulární biologie CEP GAP501/10/1740 GA ČR - Grantová agentura ČR GAP501/10/1778 GA ČR - Grantová agentura ČR GBP501/12/G090 GA ČR - Grantová agentura ČR LO1204 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora UEB-Q - RVO:61389030 UT WOS 000332051500011 DOI 10.1016/j.biotechadv.2013.12.011 Anotace Next generation sequencing (NGS) is revolutionizing genomics and is providing novel insights into genome organization, evolution and function. The number of plant genomes targeted for sequencing is rising. For the moment, however, the acquisition of full genome sequences in large genome species remains difficult, largely because the short reads produced by NGS platforms are inadequate to cope with repeat-rich DNA, which forms a large part of these genomes. The problem of sequence redundancy is compounded in polyploids, which dominate the plant kingdom. An approach to overcoming some of these difficulties is to reduce the full nuclear genome to its individual chromosomes using flow-sorting. The DNA acquired in this way has proven to be suitable for many applications, including PCR-based physical mapping, in situ hybridization, forming DNA arrays, the development of DNA markers, the construction of BAC libraries and positional cloning. Coupling chromosome sorting with NGS offers opportunities for the study of genome organization at the single chromosomal level, for comparative analyses between related species and for the validation of whole genome assemblies. Apart from the primary aim of reducing the complexity of the template, taking a chromosome-based approach enables independent teams to work in parallel, each tasked with the analysis of a different chromosome(s). Given that the number of plant species tractable for chromosome sorting is increasing, the likelihood is that chromosome genomics - the marriage of cytology and genomics - will make a significant contribution to the field of plant genetics. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2015
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