Počet záznamů: 1
Characterization of transmembrane auxin transport in Arabidopsis suspension-cultured cells
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SYSNO ASEP 0429938 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Characterization of transmembrane auxin transport in Arabidopsis suspension-cultured cells Tvůrce(i) Seifertová, Daniela (UEB-Q)
Skůpa, Petr (UEB-Q) RID
Rychtář, J. (CA)
Laňková, Martina (UEB-Q) RID, ORCID
Pařezová, Markéta (UEB-Q)
Dobrev, Petre (UEB-Q) RID, ORCID
Hoyerová, Klára (UEB-Q) RID, ORCID
Petrášek, Jan (UEB-Q) RID, ORCID
Zažímalová, Eva (UEB-Q) RID, ORCIDZdroj.dok. Journal of Plant Physiology. - : Elsevier - ISSN 0176-1617
Roč. 171, č. 6 (2014), s. 429-437Poč.str. 9 s. Jazyk dok. eng - angličtina Země vyd. DE - Německo Klíč. slova Auxin influx ; Auxin efflux ; Auxin metabolic profiling Vědní obor RIV EB - Genetika a molekulární biologie CEP GAP305/11/0797 GA ČR - Grantová agentura ČR Institucionální podpora UEB-Q - RVO:61389030 UT WOS 000333501400008 DOI 10.1016/j.jplph.2013.09.026 Anotace Polar auxin transport is a crucial process for control and coordination of plant development. Studies of auxin transport through plant tissues and organs showed that auxin is transported by a combination of phloem flow and the active, carrier-mediated cell-to-cell transport. Since plant organs and even tissues are too complex for determination of the kinetics of carrier-mediated auxin uptake and efflux on the cellular level, simplified models of cell suspension cultures are often used, and several tobacco cell lines have been established for auxin transport assays. However, there are very few data available on the specificity and kinetics of auxin transport across the plasma membrane for Arabidopsis thaliana suspension-cultured cells. In this report, the characteristics of carrier-mediated uptake (influx) and efflux for the native auxin indole-3-acetic acid and synthetic auxins, naphthalene-1-acetic and 2,4-dichlorophenoxyacetic acids (NAA and 2,4-D, respectively) in A. thaliana ecotype Landsberg erecta suspension-cultured cells (LE line) are provided. By auxin competition assays and inhibitor treatments, we show that, similarly to tobacco cells, uptake carriers have high affinity towards 2,4-D and that NAA is a good tool for studies of auxin efflux in LE cells. In contrast to tobacco cells, metabolic profiling showed that only a small proportion of NAA is metabolized in LE cells. These results show that the LE cell line is a useful experimental system for measurements of kinetics of auxin carriers on the cellular level that is complementary to tobacco cells. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2015
Počet záznamů: 1