In search of ligands and receptors of the pollen tube: the missing link in pollen tube perception

0429937 - ÚEB 2015 RIV GB eng J - Článek v odborném periodiku
Hafidh, Said - Potěšil, D. - Fíla, Jan - Feciková, Jana - Čapková, Věra - Zdráhal, Z. - Honys, David
In search of ligands and receptors of the pollen tube: the missing link in pollen tube perception.
Biochemical Society Transactions. Roč. 42, č. 2 (2014), s. 388-394. ISSN 0300-5127. E-ISSN 1470-8752
Grant CEP: GA ČR GPP501/11/P321; GA ČR(CZ) GAP501/11/1462; GA MŠMT(CZ) LD13049; GA MŠMT(CZ) ED1.1.00/02.0068
Institucionální podpora: RVO:61389030
Klíčová slova: cell-cell signalling * chemotaxis * palmitoylation
Kód oboru RIV: ED - Fyziologie
Impakt faktor: 3.194, rok: 2014

The journey undertaken by the pollen tube in angiosperms to reach the deeply embedded female gametophyte for fertilization involves persistent guidance by the female gametophyte and accurate perception of the signals by the pollen tube. Several ovule-secreted peptides have been identified. Nevertheless, there are no exact findings on how these signals are perceived by the pollen tube. As a novel approach, we have improvised a modified SIV (semi-in vivo) technique, SIV-PS (Sly pollen tube secretome), to perform gel-free LC-MS/MS for high-throughput analysis of pollen-tube-secreted proteins. Our approach has led to the identification of over 1400 protein groups. Among them are pollen-tube-secreted ligands and receptor proteins representing potential male components in perceiving ovule-emitted cues for guidance. The present article reviews the missing link in pollen tube perception and showcases the improvised SIV-PS as a tool for high-throughput and targeted study of the pollen tube secretome.
Trvalý link: http://hdl.handle.net/11104/0234901