Počet záznamů: 1
Flow Sorting and Sequencing Meadow Fescue Chromosome 4F
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SYSNO ASEP 0422267 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Flow Sorting and Sequencing Meadow Fescue Chromosome 4F Tvůrce(i) Kopecký, David (UEB-Q) RID, ORCID
Martis, M. (DE)
Čihalíková, Jarmila (UEB-Q) RID
Hřibová, Eva (UEB-Q) RID, ORCID
Vrána, Jan (UEB-Q) RID, ORCID
Bartoš, Jan (UEB-Q) RID, ORCID
Kopecká, Jitka (UEB-Q)
Cattonaro, F. (IT)
Stočes, Štěpán (UEB-Q) RID
Novák, Petr (BC-A) RID, ORCID
Neumann, Pavel (BC-A) RID, ORCID
Macas, Jiří (BC-A) RID, ORCID, SAI
Šimková, Hana (UEB-Q) RID, ORCID
Studer, B. (DK)
Asp, T. (DK)
Baird, J. H. (US)
Navrátil, Petr (UEB-Q)
Karafiátová, Miroslava (UEB-Q) RID, ORCID
Kubaláková, Marie (UEB-Q) RID
Šafář, Jan (UEB-Q) RID, ORCID
Mayer, K. (DE)
Doležel, Jaroslav (UEB-Q) RID, ORCIDZdroj.dok. Plant Physiology. - : Oxford University Press - ISSN 0032-0889
Roč. 163, č. 3 (2013), s. 1323-1337Poč.str. 15 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova SATELLITE DNA-SEQUENCES ; FESTUCA-PRATENSIS ; LOLIUM-MULTIFLORUM Vědní obor RIV EB - Genetika a molekulární biologie CEP GAP501/11/0504 GA ČR - Grantová agentura ČR OC10037 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora UEB-Q - RVO:61389030 ; BC-A - RVO:60077344 UT WOS 000326520900020 DOI 10.1104/pp.113.224105 Anotace The analysis of large genomes is hampered by a high proportion of repetitive DNA, which makes the assembly of short sequence reads difficult. This is also the case in meadow fescue (Festuca pratensis), which is known for good abiotic stress resistance and has been used in intergeneric hybridization with ryegrasses (Lolium spp.) to produce Festulolium cultivars. In this work, we describe a new approach to analyze the large genome of meadow fescue, which involves the reduction of sample complexity without compromising information content. This is achieved by dissecting the genome to smaller parts: individual chromosomes and groups of chromosomes. As the first step, we flow sorted chromosome 4F and sequenced it by Illumina with approximately 503 coverage. This provided, to our knowledge, the first insight into the composition of the fescue genome, enabled the construction of the virtual gene order of the chromosome, and facilitated detailed comparative analysis with the sequenced genomes of rice (Oryza sativa), Brachypodium distachyon, sorghum (Sorghum bicolor), and barley (Hordeum vulgare). Using GenomeZipper, we were able to confirm the collinearity of chromosome 4F with barley chromosome 4H and the long arm of chromosome 5H. Several new tandem repeats were identified and physically mapped using fluorescence in situ hybridization. They were found as robust cytogenetic markers for karyotyping of meadow fescue and ryegrass species and their hybrids. The ability to purify chromosome 4F opens the way for more efficient analysis of genomic loci on this chromosome underlying important traits, including freezing tolerance. Our results confirm that next-generation sequencing of flow-sorted chromosomes enables an overview of chromosome structure and evolution at a resolution never achieved before. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2014
Počet záznamů: 1