Proteome and metabolome profiling of cytokinin action in Arabidopsis identifying both distinct and similar responses to cytokinin down- and up-regulation >

0422255 - BFÚ 2014 RIV GB eng J - Článek v odborném periodiku
Černý, M. - Kuklová, A. - Hoehenwarter, W. - Fragner, L. - Novák, Ondřej - Rotková, Gabriela - Jedelský, P. - Žáková, K. - Šmehilová, M. - Strnad, Miroslav - Weckwerth, W. - Brzobohatý, Břetislav
Proteome and metabolome profiling of cytokinin action in Arabidopsis identifying both distinct and similar responses to cytokinin down- and up-regulation >.
Journal of Experimental Botany. Roč. 64, č. 14 (2013), s. 4193-4206. ISSN 0022-0957. E-ISSN 1460-2431
Grant CEP: GA ČR(CZ) GA206/09/2062; GA MŠMT(CZ) ED1.1.00/02.0068
Grant ostatní: GA ČR(CZ) GAP305/12/2144
Výzkumný záměr: CEZ:AV0Z50040702
Institucionální podpora: RVO:68081707
Klíčová slova: INDUCIBLE GENE-EXPRESSION * BETA-GLUCOSIDASE * PLANT-GROWTH
Kód oboru RIV: BO - Biofyzika; EF - Botanika (UEB-Q)
Impakt faktor: 5.794, rok: 2013

In plants, numerous developmental processes are controlled by cytokinin (CK) levels and their ratios to levels of other hormones. While molecular mechanisms underlying the regulatory roles of CKs have been intensely researched, proteomic and metabolomic responses to CK deficiency are unknown. Transgenic Arabidopsis seedlings carrying inducible barley cytokinin oxidase/dehydrogenase (CaMV35S > GR > HvCKX2) and agrobacterial isopentenyl transferase (CaMV35S > GR > ipt) constructs were profiled to elucidate proteome- and metabolome-wide responses to down- and up-regulation of CK levels, respectively. Proteome profiling identified > 1100 proteins, 155 of which responded to HvCKX2 and/or ipt activation, mostly involved in growth, development, and/or hormone and light signalling. The metabolome profiling covered 79 metabolites, 33 of which responded to HvCKX2 and/or ipt activation, mostly amino acids, carbohydrates, and organic acids.
Trvalý link: http://hdl.handle.net/11104/0228496