Počet záznamů: 1
Gene Networks and Chromatin and Transcriptional Regulation of the Phaseolin Promoter in Arabidopsis
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SYSNO ASEP 0399321 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Gene Networks and Chromatin and Transcriptional Regulation of the Phaseolin Promoter in Arabidopsis Tvůrce(i) Sundaram, S. (US)
Kertbundit, Sunee (UEB-Q)
Shakirov, E. (US)
Iyer, L.M. (US)
Juříček, Miloslav (UEB-Q) RID, ORCID
Hall, T.C. (US)Zdroj.dok. Plant Cell. - : Oxford University Press - ISSN 1040-4651
Roč. 25, č. 7 (2013), s. 2601-2617Poč.str. 17 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova SEED MATURATION GENES ; ABSCISIC-ACID ; RNA-SEQ Vědní obor RIV EB - Genetika a molekulární biologie CEP ME10038 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy CEZ AV0Z50380511 - UEB-Q (2005-2011) UT WOS 000323650900019 DOI 10.1105/tpc.113.112714 Anotace The complete lack of seed storage protein expression in vegetative tissues and robust expression during embryogenesis makes seed development an ideal system to study tissue-specific expression of genes. The promoter for the Phaseolin (phas) gene, which encodes the major seed storage protein in bean (Phaseolus vulgaris), is activated in two sequential steps: Phaseolus vulgaris ABI3-like factor (Pv-ALF)-dependent potentiation and abscisic acid-mediated activation. In this study, a heterologous in vivo Pv-ALF/phas-GUS (for beta-glucuronidase) expression system in transgenic Arabidopsis thaliana leaves was used in conjunction with the powerful RNA-Seq approach to capture transcriptional landscapes of phas promoter expression. Remarkably, expression of over 1300 genes from 11 functional categories coincided with changes in the transcriptional status of the phas promoter. Gene network analysis of induced genes and artificial microRNA-mediated loss-of-function genetic assays identified transcriptional regulators RINGLET 2 (RLT2) and AINTEGUMENTA-LIKE 5 (AIL5) as being essential for phas transcription. Pv-ALF binding to the RLT2 and AIL5 promoter regions was confirmed by electrophoretic mobility shift assay. RLT2 and AIL5 knockdown lines displayed reduced expression of several endogenous seed genes, suggesting that these factors are involved in activation of endogenous Arabidopsis seed storage gene expression. Overall, the identification of these key factors involved in phas activation provides important insight into the two-step transcriptional regulation of seed-specific gene expression. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2014
Počet záznamů: 1