Počet záznamů: 1
Assessment of the role of meta-topolins on in vitro produced phenolics and acclimatization competence of micropropagated 'Williams' banana
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SYSNO ASEP 0384334 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Assessment of the role of meta-topolins on in vitro produced phenolics and acclimatization competence of micropropagated 'Williams' banana Tvůrce(i) Aremu, A.O. (ZA)
Bairu, M. W. (ZA)
Szüčová, Lucie (UEB-Q) RID, ORCID
Doležal, Karel (UEB-Q) RID, ORCID
Finnie, J.F. (ZA)
van Staden, J. (ZA)Zdroj.dok. Acta Physiologiae Plantarum. - : Springer - ISSN 0137-5881
Roč. 34, č. 6 (2012), s. 2265-2273Poč.str. 9 s. Jazyk dok. eng - angličtina Země vyd. PL - Polsko Klíč. slova Cytokinins ; Chlorophyll ; Phenolics Vědní obor RIV EB - Genetika a molekulární biologie CEP QI92A247 GA MZe - Ministerstvo zemědělství CEZ AV0Z50380511 - UEB-Q (2005-2011) UT WOS 000310165900020 DOI 10.1007/s11738-012-1027-6 Anotace The effects of five topolins (meta-Topolin, mT; meta-Topolin riboside, mTR; meta-Methoxy topolin, MemT; meta-Methoxy topolin riboside, MemTR and meta-Methoxy topolin 9-tetrahydropyran-2-yl, MemTTHP) on the phenolic content and subsequent acclimatization potential of micropropagated 'Williams' bananas were compared to benzyladenine (BA). Sterile shoot-tip explants were cultured on modified Murashige and Skoog (MS) media containing 10, 20 or 30 mu M of the above aromatic cytokinins (CKs) for 42 days. Phenolic contents were quantified spectrophotometrically. Growth parameters and photosynthetic pigments of the greenhouse-acclimatized plants were determined after 5 months. Total phenolic levels were highest in 10 mu M mT-treated plantlets within the aerial parts and 30 mu M MemTTHP for the underground parts. In the underground parts, 10 mu M mT resulted in the production of the highest amount of proanthocyanidins which was approximately five-fold higher than in the control plants. Furthermore, 10 mu M MemTTHP-treated plantlets had significantly higher total flavonoids (30.1 +/- A 0.24 mg CE/g DW) within the aerial parts. Plantlets regenerated using MemT, MemTR and MemTTHP had significantly longer roots and better shoot/root ratios compared to the control and BA-treated plants. In terms of root fresh weight, it was significantly higher in MemT-treated plantlets than in the control and BA treatments. Chlorophyll a/b ratio was significantly improved with the use of MemT, mTR and mT compared to control. Current findings indicate the potential of topolins in stimulating the accumulation of phenolic compounds in micropropagated plantlets. In view of the importance of plant secondary metabolites, their substantial accumulation probably enhanced the acclimatization and subsequent ex vitro survival of the micropropagated plantlets. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2013
Počet záznamů: 1