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Surface Plasmon Resonance Biosensor for Rapid Label-Free Detection of Microribonucleic Acid at Subfemtomole Level
- 1.0358219 - ÚFE 2011 RIV US eng J - Článek v odborném periodiku
Šípová, Hana - Zhang, S.-Ch. - Dudley, A.M. - Galas, D. - Wang, K. - Homola, Jiří
Surface Plasmon Resonance Biosensor for Rapid Label-Free Detection of Microribonucleic Acid at Subfemtomole Level.
Analytical Chemistry. Roč. 82, č. 24 (2010), s. 10110-10115. ISSN 0003-2700. E-ISSN 1520-6882
Grant CEP: GA AV ČR KAN200670701
Výzkumný záměr: CEZ:AV0Z20670512
Klíčová slova: surface plasmon resonance * microRNA * cancer diagnostics * biosensor
Kód oboru RIV: JB - Senzory, čidla, měření a regulace
Impakt faktor: 5.874, rok: 2010
MicroRNAs (miRNAs) present an important target for medical diagnostics of various serious diseases. We report a novel method for rapid and sensitive miRNA detection and quantization using high-performance portable surface plasmon resonance (SPR) sensor combined with a DNA*RNA antibody-based assay. The new approach allows detecting miRNA at levels down to high attomoles in less than 30 minutes. The methodology is found to yield results which agree well with established methods for miRNA detection.
Trvalý link: http://hdl.handle.net/11104/0196316
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