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Plant Phosphatidylcholine-Hydrolyzing Phospholipases C NPC3 and NPC4 with Roles in Root Development and Brassinolide Signaling in Arabidopsis thaliana
- 1.0349937 - ÚEB 2011 RIV GB eng J - Článek v odborném periodiku
Wimalasekera, R. - Pejchar, Přemysl - Holk, A. - Martinec, Jan - Scherer, G.F.E.
Plant Phosphatidylcholine-Hydrolyzing Phospholipases C NPC3 and NPC4 with Roles in Root Development and Brassinolide Signaling in Arabidopsis thaliana.
Molecular Plant. Roč. 3, č. 3 (2010), s. 610-625. ISSN 1674-2052. E-ISSN 1752-9867
Grant CEP: GA MŠMT(CZ) LC06034; GA ČR GA522/07/1614
Výzkumný záměr: CEZ:AV0Z50380511
Klíčová slova: Auxin * brassinolide signaling * phosphate deficiency
Kód oboru RIV: ED - Fyziologie
Impakt faktor: 4.296, rok: 2010
Phosphatidylcholine-hydrolyzing phospholipase C (PC-PLC) catalyzes the hydrolysis of phosphatidylcholine (PC) to generate phosphocholine and diacylglycerol (DAG). PC-PLC has a long tradition in animal signal transduction to generate DAG as a second messenger besides the classical phosphatidylinositol splitting phospholipase C (PI-PLC). Based on amino acid sequence similarity to bacterial PC-PLC, six putative PC-PLC genes (NPC1 to NPC6) were identified in the Arabidopsis genome. RT-PCR analysis revealed overlapping expression pattern of NPC genes in root, stem, leaf, flower, and silique. In auxin-treated P-NPC3:GUS and P-NPC4:GUS seedlings, strong increase of GUS activity was visible in roots, leaves, and shoots and, to a weaker extent, in brassinolide-treated (BL) seedlings. P-NPC4:GUS seedlings also responded to cytokinin with increased GUS activity in young leaves. Compared to wild-type, T-DNA insertional knockouts npc3 and npc4 showed shorter primary roots and lower lateral root density.
Trvalý link: http://hdl.handle.net/11104/0190058
Název souboru Staženo Velikost Komentář Verze Přístup 2010_Wimalasekera_MOLECULAR PLANT_610.pdf 4 1.7 MB Jiná povolen
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