Počet záznamů: 1
Fiber-type composition and 3D capillary analysis of the human splenius capitis muscle
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SYSNO ASEP 0619372 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Fiber-type composition and 3D capillary analysis of the human splenius capitis muscle Tvůrce(i) Pollak, N. (SI)
Ugwoke, Ch. K. (SI)
Umek, N. (SI)
Alibegović, A. (SI)
Janáček, Jiří (FGU-C) RID, ORCID
Radochová, Barbora (FGU-C) RID, ORCID
Cvetko, E. (SI)Zdroj.dok. Biomolecules and Biomedicine - ISSN 2831-0896
Roč. 25, č. 6 (2025), s. 1351-1361Poč.str. 11 s. Jazyk dok. eng - angličtina Země vyd. BA - Bosna a Hercegovina Klíč. slova skeletal muscle ; splenius capitis muscle ; vastus lateralis muscle ; capillary network ; myosin heavy chain isoforms ; MyHC ; 3D image analysis ; human Obor OECD Anatomy and morphology (plant science to be 1.6) CEP GF22-02756K GA ČR - Grantová agentura ČR EF18_046/0016045 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Výzkumná infrastruktura Czech-BioImaging III - 90250 - Ústav molekulární genetiky AV ČR, v. v. i. Způsob publikování Open access Institucionální podpora FGU-C - RVO:67985823 UT WOS 001310873000001 EID SCOPUS 105004402854 DOI https://doi.org/10.17305/bb.2024.10787 Anotace Despite the significance of neck muscles in musculoskeletal disorders, their microscopic anatomy remains poorly characterized. This study examined the splenius capitis muscle, focusing on its fiber-type composition, fiber size, and capillary network characteristics. For comparison and validation, the vastus lateralis muscle was also analyzed. Muscle samples from 13 young male subjects (mean age ± SD: 35.7 ± 8.6 years) were collected within 24-h post-mortem during autopsy. Myosin heavy chain (MyHC) isoform expression was characterized immunohistochemically in 10 μm sections, while the capillary network architecture was assessed in 100 μm sections. Immunofluorescence staining, confocal microscopy, and 3D image analysis were employed to quantify capillary tortuosity, anisotropy, branch density (Br dens), and the length of capillaries per muscle volume (LV), per muscle fiber length (LL), per fiber surface area (LS), and per fiber volume (LVf). Compared to the vastus lateralis muscle, the splenius capitis muscle had a higher percentage of type 1 fibers (51.2% vs 39.7%), fewer type 2a fibers (16.2% vs 31.4%), and smaller fiber diameters (35.5–40.9 μm vs 47–56.1 μm). It also displayed lower Br dens (P = 0.0069), higher anisotropy (P = 0.0004), and lower LL (P < 0.0001) but higher LVf (P = 0.0486). In the splenius capitis muscle, body mass index (BMI) negatively correlated with LV (P = 0.0155), LS (P = 0.0091), LVf (P = 0.0137), and anisotropy (P = 0.0425), and positively correlated with tortuosity (P = 0.0473), indicating a reduction in the capillary network. In the vastus lateralis muscle, only LV (P = 0.0161) decreased with high BMI. This study characterized the fiber-type composition, fiber size, and 3D capillary network of the splenius capitis muscle, establishing a baseline for investigations into pathological muscle alterations. Pracoviště Fyziologický ústav Kontakt Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Rok sběru 2026 Elektronická adresa https://doi.org/10.17305/bb.2024.10787
Počet záznamů: 1