Počet záznamů: 1
Topical siRNA therapy of diabetic-like wound healing
- 1.
SYSNO ASEP 0616560 Druh ASEP J - Článek v odborném periodiku Zařazení RIV Záznam nebyl označen do RIV Poddruh J Článek ve WOS Název Topical siRNA therapy of diabetic-like wound healing Tvůrce(i) Neuhöferová, Eva (MBU-M)
Kindermann, Marek (UOCHB-X) ORCID
Buzgo, Matej (UEM-P)
Vocetková, Karolína (UEM-P) ORCID
Pánek, D. (CZ)
Cígler, Petr (UOCHB-X) RID, ORCID
Benson, Veronika (MBU-M) RID, ORCIDZdroj.dok. Journal of Materials Chemistry B - ISSN 2050-750X
Roč. 13, č. 3 (2024), s. 1037-1051Poč.str. 15 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova fluorescent nanodiamonds ; controlled-release ; delivery ; degradation ; nanofibers ; dressings ; membranes Obor OECD Microbiology CEP GA23-04876S GA ČR - Grantová agentura ČR TH90010001 GA TA ČR - Technologická agentura ČR EH22_008/0004558 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora MBU-M - RVO:61388971 ; UOCHB-X - RVO:61388963 ; UEM-P - RVO:68378041 UT WOS 001369613300001 EID SCOPUS 85211064871 DOI https://doi.org/10.1039/d4tb01547a Anotace Non-healing wounds are a serious complication in diabetic patients. One of the detrimental factors contributing to limited wound healing is the accumulation of metalloproteinase-9 (MMP-9) in the wound. Selective inhibition of MMP-9 is one of the established therapeutic targets for diabetic wound healing. Here, a functional and biocompatible wound dressing is developed to enable a controlled release of a traceable vector loaded with the antisense siRNA against MMP-9 in the wound. The dressing consists of degradable polymer nanofibers embedded with a vector nanosystem polymer-coated fluorescent nanodiamonds optimized for the binding of siRNA and colloidal stability of nanodiamond-siRNA complexes in a physiological environment. The developed dressing is tested on murine fibroblasts and also applied to wounds in a diabetic murine model to evaluate its suitability in terms of in vivo toxicity, biological efficacy, and handling. The treatment results in significant local inhibition of MMP-9 and a shortening of the wound healing time. The scar formation in treated diabetic-like mice becomes comparable with that in non-treated diabetes-free mice. Our results suggest that the application of our biocompatible dressing loaded with a non-toxic vector nanosystem is an effective and promising approach to gene therapy of non-healing wounds. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2026 Elektronická adresa https://pubs.rsc.org/en/content/articlelanding/2025/tb/d4tb01547a
Počet záznamů: 1