Počet záznamů: 1  

Topical siRNA therapy of diabetic-like wound healing

  1. 1.
    SYSNO ASEP0616560
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVZáznam nebyl označen do RIV
    Poddruh JČlánek ve WOS
    NázevTopical siRNA therapy of diabetic-like wound healing
    Tvůrce(i) Neuhöferová, Eva (MBU-M)
    Kindermann, Marek (UOCHB-X) ORCID
    Buzgo, Matej (UEM-P)
    Vocetková, Karolína (UEM-P) ORCID
    Pánek, D. (CZ)
    Cígler, Petr (UOCHB-X) RID, ORCID
    Benson, Veronika (MBU-M) RID, ORCID
    Zdroj.dok.Journal of Materials Chemistry B - ISSN 2050-750X
    Roč. 13, č. 3 (2024), s. 1037-1051
    Poč.str.15 s.
    Jazyk dok.eng - angličtina
    Země vyd.GB - Velká Británie
    Klíč. slovafluorescent nanodiamonds ; controlled-release ; delivery ; degradation ; nanofibers ; dressings ; membranes
    Obor OECDMicrobiology
    CEPGA23-04876S GA ČR - Grantová agentura ČR
    TH90010001 GA TA ČR - Technologická agentura ČR
    EH22_008/0004558 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
    Způsob publikováníOpen access
    Institucionální podporaMBU-M - RVO:61388971 ; UOCHB-X - RVO:61388963 ; UEM-P - RVO:68378041
    UT WOS001369613300001
    EID SCOPUS85211064871
    DOI https://doi.org/10.1039/d4tb01547a
    AnotaceNon-healing wounds are a serious complication in diabetic patients. One of the detrimental factors contributing to limited wound healing is the accumulation of metalloproteinase-9 (MMP-9) in the wound. Selective inhibition of MMP-9 is one of the established therapeutic targets for diabetic wound healing. Here, a functional and biocompatible wound dressing is developed to enable a controlled release of a traceable vector loaded with the antisense siRNA against MMP-9 in the wound. The dressing consists of degradable polymer nanofibers embedded with a vector nanosystem polymer-coated fluorescent nanodiamonds optimized for the binding of siRNA and colloidal stability of nanodiamond-siRNA complexes in a physiological environment. The developed dressing is tested on murine fibroblasts and also applied to wounds in a diabetic murine model to evaluate its suitability in terms of in vivo toxicity, biological efficacy, and handling. The treatment results in significant local inhibition of MMP-9 and a shortening of the wound healing time. The scar formation in treated diabetic-like mice becomes comparable with that in non-treated diabetes-free mice. Our results suggest that the application of our biocompatible dressing loaded with a non-toxic vector nanosystem is an effective and promising approach to gene therapy of non-healing wounds.
    PracovištěMikrobiologický ústav
    KontaktEliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
    Rok sběru2026
    Elektronická adresahttps://pubs.rsc.org/en/content/articlelanding/2025/tb/d4tb01547a
Počet záznamů: 1  

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