Počet záznamů: 1  

Molecular and morphological characterisation of larvae of the genus Diamesa Meigen, 1835 (Diptera: Chironomidae) in Alpine streams (Ötztal Alps, Austria)

  1. 1.
    SYSNO ASEP0605476
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    Poddruh JČlánek ve WOS
    NázevMolecular and morphological characterisation of larvae of the genus Diamesa Meigen, 1835 (Diptera: Chironomidae) in Alpine streams (Ötztal Alps, Austria)
    Tvůrce(i) Dvořák, M. (CZ)
    Dittmann, I.L. (AT)
    Pedrini-Martha, V. (AT)
    Hamerlik, L. (SK)
    Bitušík, P. (SK)
    Stuchlík, Evžen (BC-A) RID, ORCID
    Vondrák, D. (CZ)
    Fuereder, L. (AT)
    Lackner, R. (AT)
    Celkový počet autorů9
    Číslo článkue0298367
    Zdroj.dok.PLoS ONE. - : Public Library of Science - ISSN 1932-6203
    Roč. 19, č. 2 (2024)
    Poč.str.15 s.
    Jazyk dok.eng - angličtina
    Země vyd.US - Spojené státy americké
    Klíč. slovainternal transcribed spacer ; oxidase subunit-i ; ribosomal dna
    Vědní obor RIVEG - Zoologie
    Obor OECDEntomology
    CEPGF20-00892L GA ČR - Grantová agentura ČR
    Způsob publikováníOpen access
    Institucionální podporaBC-A - RVO:60077344
    UT WOS001164173200020
    EID SCOPUS85185232391
    DOI https://doi.org/10.1371/journal.pone.0298367
    AnotaceDiamesa species (Diptera, Chironomidae) are widely distributed in freshwater ecosystems, and their life cycles are closely linked to environmental variables such as temperature, water quality, and sediment composition. Their sensitivity to environmental changes, particularly in response to pollution and habitat alterations, makes them valuable indicators of ecosystem health. The challenges associated with the morphological identification of larvae invoke the use of DNA barcoding for species determination. The mitochondrial cytochrome oxidase subunit I (COI) gene is regularly used for species identification but faces limitations, such as similar sequences in closely related species. To overcome this, we explored the use of the internal transcribed spacers (ITS) region in addition to COI for Diamesa larvae identification. Therefore, this study employs a combination of molecular markers alongside traditional morphological identification to enhance species discrimination. In total, 129 specimens were analysed, of which 101 were sampled from a glacier-fed stream in Rotmoostal, and the remaining 28 from spring-fed streams in the neighbouring valleys of Konigstal and Timmelstal. This study reveals the inadequacy of utilizing single COI or ITS genes for comprehensive species differentiation within the genus Diamesa. However, the combined application of COI and ITS markers significantly enhances species identification resolution, surpassing the limitations faced by traditional taxonomists. Notably, this is evident in cases involving morphologically indistinguishable species, such as Diamesa latitarsis and Diamesa modesta. It highlights the potential of employing a multi-marker approach for more accurate and reliable Diamesa species identification. This method can be a powerful tool for identifying Diamesa species, shedding light on their remarkable adaptations to extreme environments and the impacts of environmental changes on their populations.
    PracovištěBiologické centrum (od r. 2006)
    KontaktDana Hypšová, eje@eje.cz, Tel.: 387 775 214
    Rok sběru2025
    Elektronická adresahttps://doi.org/10.1371/journal.pone.0298367
Počet záznamů: 1  

  Tyto stránky využívají soubory cookies, které usnadňují jejich prohlížení. Další informace o tom jak používáme cookies.