Počet záznamů: 1
Dual thermo- and pH-responsive polymer nanoparticle assemblies for potential stimuli-controlled drug delivery
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SYSNO ASEP 0604832 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Dual thermo- and pH-responsive polymer nanoparticle assemblies for potential stimuli-controlled drug delivery Tvůrce(i) Pytlíková, Sára (UMCH-V)
Konefal, Rafal (UMCH-V) RID, ORCID
Pola, Robert (UMCH-V) RID, ORCID
Braunová, Alena (UMCH-V) RID
Lobaz, Volodymyr (UMCH-V) RID, ORCID
Šlouf, Miroslav (UMCH-V) RID, ORCID
Beneš, Hynek (UMCH-V) RID, ORCID
Starenko, Daniil (MBU-M)
Běhalová, Kateřina (MBU-M) ORCID
Kovář, Marek (MBU-M) RID, ORCID
Etrych, Tomáš (UMCH-V) RID, ORCID
Laga, Richard (UMCH-V) RID, ORCID
Pechar, Michal (UMCH-V) RID, ORCIDZdroj.dok. ACS Applied Bio Materials. - : American Chemical Society - ISSN 2576-6422
Roč. 8, č. 1 (2025), s. 271-284Poč.str. 14 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova thermoresponsive polymers ; pH-sensitive polymers ; self-assembling block copolymers Vědní obor RIV CD - Makromolekulární chemie Obor OECD Polymer science Vědní obor RIV – spolupráce Mikrobiologický ústav - Farmakologie a lékárnická chemie CEP LUAUS24239 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy LX22NPO5102 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy LM2023053 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora UMCH-V - RVO:61389013 ; MBU-M - RVO:61388971 UT WOS 001375023100001 EID SCOPUS 85211621102 DOI https://doi.org/10.1021/acsabm.4c01167 Anotace The development of stimuli-responsive drug delivery systems enables targeted delivery and environment-controlled drug release, thereby minimizing off-target effects and systemic toxicity. We prepared and studied tailor-made dual-responsive systems (thermo- and pH-) based on synthetic diblock copolymers consisting of a fully hydrophilic block of poly[N-(1,3-dihydroxypropyl)methacrylamide] (poly(DHPMA)) and a thermoresponsive block of poly[N-(2,2-dimethyl-1,3-dioxan-5-yl)methacrylamide] (poly(DHPMA-acetal)) as drug delivery and smart stimuli-responsive materials. The copolymers were designed for eventual medical application to be fully soluble in aqueous solutions at 25 °C. However, they form well-defined nanoparticles with hydrodynamic diameters of 50–800 nm when heated above the transition temperature of 27–31 °C. This temperature range is carefully tailored to align with the human body’s physiological conditions. The formation of the nanoparticles and their subsequent decomposition was studied using dynamic light scattering (DLS), transmission electron microscopy (TEM), isothermal titration calorimetry (ITC), and nuclear magnetic resonance (NMR). 1H NMR studies confirmed that after approximately 20 h of incubation at pH 5, which closely mimics tumor microenvironment, approximately 40% of the acetal groups were hydrolyzed, and the thermoresponsive behavior of the copolymers was lost. This smart polymer response led to disintegration of the supramolecular structures, possibly releasing the therapeutic cargo. By tuning the transition temperature to the values relevant for medical applications, we ensure precise and effective drug release. In addition, our systems did not exhibit any cytotoxicity against any of the three cell lines. Our findings underscore the immense potential of these nanoparticles as eventual advanced drug delivery systems, especially for cancer therapy. Pracoviště Ústav makromolekulární chemie Kontakt Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Rok sběru 2026 Elektronická adresa https://pubs.acs.org/doi/10.1021/acsabm.4c01167
Počet záznamů: 1