0604691 - FGÚ 2026 RIV NL eng J - Článek v odborném periodiku
Slepička, P. - Kasálková-Slepičková, N. - Musílková, Jana - Bačáková, Lucie - Frýdlova, B. - Sajdl, P. - Kolská, Z. - Rebollar, E. - Švorčík, V.
PLLA honeycombs activated by plasma and high-energy excimer laser for stem cell support.
Applied Surface Science Advances. Roč. 25, January (2025), č. článku 100662. ISSN 2666-5239. E-ISSN 2666-5239
Grant CEP: GA MZd(CZ) NU20-08-00208
Institucionální podpora: RVO:67985823
Klíčová slova: honeycomb pattern * organic coating * laser treatment * biopolymer * morphology * cytocompatibility
Obor OECD: Biomaterials (as related to medical implants, devices, sensors)
Impakt faktor: 7.5, rok: 2023 ; AIS: 1.060, rok: 2023
Způsob publikování: Open access
Web výsledku:
https://doi.org/10.1016/j.apsadv.2024.100662DOI: https://doi.org/10.1016/j.apsadv.2024.100662

In this study, we constructed and activated honeycomb structures on perfluorinated substrates subjected to KrF laser treatment (wavelength 248 nm). We selected the biopolymer poly L-lactic acid (PLLA) as the honeycomb material, which was dissolved in a mixture of chloroform/methanol. A micropattern of a plasma-treated perfluorethyleneperopylene (FEP) substrate was prepared by improved phase separation during dip-coating. The PLLA micropattern was subsequently treated with an excimer laser with a laser fluence of 10 mJ.cm-2 and with a different number of laser pulses. Alternatively, plasma exposure can be used as a secondary treatment. The surface morphologies of the pristine and laser-treated PLLA patterns were studied using atomic force and scanning electron microscopy. The surface chemistry was analyzed using energy-dispersive spectroscopy and Xray photoelectron spectroscopy. In addition, the metabolic activity of adipose stem cells was evaluated using the MTS test, and cell numbers in selected samples were determined. The morphology of cells growing in the honeycomb-like pattern was studied in detail using fluorescence microscopy. In all, we used a combination of honeycomb pattern (HCP) laser treatment and plasma treatment to construct an optimal scaffold for adipose stem cell culture.
Trvalý link: https://hdl.handle.net/11104/0363472
Vědecká data: Zenodo