Počet záznamů: 1
Protamine 2 deficiency results in Septin 12 abnormalities
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SYSNO ASEP 0601325 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Protamine 2 deficiency results in Septin 12 abnormalities Tvůrce(i) Šanovec, Ondřej (BTO-N) RID, ORCID
Frolíková, Michaela (BTO-N) ORCID
Kraus, Veronika (BTO-N) RID, ORCID
Vondráková, Jana (BTO-N) ORCID, RID
Qasemi, Maryam (BTO-N) RID, ORCID
Spěváková, Daniela (BTO-N) RID
Šimoník, Ondřej (BTO-N) RID, ORCID
Moritz, L. (US)
Caswell, D. L. (US)
Liška, F. (CZ)
Děd, Lukáš (BTO-N) RID, ORCID
Černý, Jiří (BTO-N) RID, ORCID
Avidor-Reiss, T. (US)
Hammoud, S. S. (US)
Schorle, H. (DE)
Postlerová, Pavla (BTO-N) ORCID, RID
Steger, K. (DE)
Komrsková, Kateřina (BTO-N) ORCID, RIDCelkový počet autorů 18 Číslo článku 1447630 Zdroj.dok. Frontiers in Cell and Developmental Biology. - : Frontiers Research Foundation - ISSN 2296-634X
Roč. 12, OCT 25 2024 (2024)Poč.str. 23 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova sperm ; mouse ; Lamin ; annulus ; asthenozoospermia ; organization ; DNA, ; sperm immotility Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Reproductive biology (medical aspects to be 3) CEP GA23-06591S GA ČR - Grantová agentura ČR GC20-20217J GA ČR - Grantová agentura ČR ED1.1.00/02.0109 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy EF16_013/0001775 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy LM2018129 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy LM2023050 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora BTO-N - RVO:86652036 UT WOS 001349849600001 EID SCOPUS 85208633807 DOI https://doi.org/10.3389/fcell.2024.1447630 Anotace There is a well-established link between abnormal sperm chromatin states and poor motility, however, how these two processes are interdependent is unknown. Here, we identified a possible mechanistic insight by showing that Protamine 2, a nuclear DNA packaging protein in sperm, directly interacts with cytoskeletal protein Septin 12, which is associated with sperm motility. Septin 12 has several isoforms, and we show, that in the Prm2/- sperm, the short one (Mw 36 kDa) is mis-localized, while two long isoforms (Mw 40 and 41 kDa) are unexpectedly lost in Prm2/- sperm chromatin-bound protein fractions. Septin 12 co-immunoprecipitated with Protamine 2 in the testicular cell lysate of WT mice and with Lamin B1/2/3 in co-transfected HEK cells despite we did not observe changes in Lamin B2/B3 proteins or SUN4 expression in Prm2/- testes. Furthermore, the Prm2/- sperm have on average a smaller sperm nucleus and aberrant acrosome biogenesis. In humans, patients with low sperm motility (asthenozoospermia) have imbalanced histone-protamine 1/2 ratio, modified levels of cytoskeletal proteins and we detected retained Septin 12 isoforms (Mw 40 and 41 kDa) in the sperm membrane, chromatin-bound and tubulin/mitochondria protein fractions. In conclusion, our findings present potential interaction between Septin 12 and Protamine 2 or Lamin B2/3 and describe a new connection between their expression and localization, contributing likely to low sperm motility and morphological abnormalities. Pracoviště Biotechnologický ústav Kontakt Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Rok sběru 2025 Elektronická adresa https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2024.1447630/full
Počet záznamů: 1