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Exploring the Siderophore Portfolio for Mass Spectrometry-Based Diagnosis of Scedosporiosis and Lomentosporiosis
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SYSNO ASEP 0600389 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Exploring the Siderophore Portfolio for Mass Spectrometry-Based Diagnosis of Scedosporiosis and Lomentosporiosis Tvůrce(i) Houšť, Jiří (MBU-M) ORCID
Palyzová, Andrea (MBU-M) RID, ORCID
Pluháček, Tomáš (MBU-M) ORCID
Novák, Jiří (MBU-M) RID, ORCID
Marešová, Helena (MBU-M) RID, ORCID
Hubáček, P. (CZ)
Dobiáš, R. (CZ)
Stevens, D. A. (US)
Guegan, H. (FR)
Gangneux, J. (FR)
Havlíček, Vladimír (MBU-M) RID, ORCIDZdroj.dok. ACS Omega. - : American Chemical Society - ISSN 2470-1343
Roč. 9, č. 44 (2024), s. 44815-44824Poč.str. 10 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova iron acquisition ; prolificans ; database ; apiospermum ; infection ; peptides Obor OECD Microbiology CEP EH22_008/0004597 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora MBU-M - RVO:61388971 UT WOS 001340177000001 EID SCOPUS 85207278731 DOI https://doi.org/10.1021/acsomega.4c08257 Anotace Scedosporium apiospermum and Lomentospora prolificans secrete siderophores (iron scavengers) during hyphal proliferation. Siderophores are virulence factors and potential clinical biomarkers of invasive scedosporiosis and lomentosporiosis. Both strains secreted a uniform spectrum of siderophores, including coprogen B (CopB), N alpha-methyl-coprogen B, dimethyl-coprogen, and ferricrocin, with N alpha-methyl-coprogen B being the fastest secreted and most abundant coprogen. Under iron and zinc restriction, reflecting a nutrient-limited host environment, L. prolificans secreted 45 times more CopB than did S. apiospermum, presumably contributing to its higher virulence. This robust mobilization of CopB was further enhanced by zinc surplus. Additionally, two novel cyclic peptides, Scedocyclin A and B, were characterized inScedosporium boydii using the de novo sequencing tool CycloBranch. Utilizing matrix-assisted laser desorption/ionization, the portfolio of coprogens detected had limits of detection and quantitation of 4.9 and 14.6 fmol/spot in complex matrices, respectively, making them strong candidates for the next-generation, routine diagnosis of invasive scedosporiosis and lomentosporiosis through the Biotyper siderotyping. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2025 Elektronická adresa https://pubs.acs.org/doi/epdf/10.1021/acsomega.4c08257?ref=article_openPDF
Počet záznamů: 1