0599437 - MBÚ 2025 RIV US eng J - Článek v odborném periodiku
Brodsky, Katerina - Petránková, Barbora - Petrásková, Lucie - Pelantová, Helena - Křen, Vladimír - Valentová, Kateřina - Bojarová, Pavla
New Bacterial Aryl Sulfotransferases: Effective Tools for Sulfation of Polyphenols.
Journal of Agricultural and Food Chemistry. Roč. 72, č. 40 (2024), s. 22208-22216. ISSN 0021-8561. E-ISSN 1520-5118
Grant CEP: GA ČR(CZ) GA23-04654S; GA MZd(CZ) NU21-02-00135; GA MŠMT(CZ) EH22_008/0004597
Institucionální podpora: RVO:61388971
Klíčová slova: arylsulfate sulfotransferase * enzymatic sulfation * flavonoids * system * metabolites * quercetin * mechanism * aryl sulfotransferase * enzymaticsulfation * kaempferol sulfate * metabolite * polyphenol
Obor OECD: Biochemistry and molecular biology
Impakt faktor: 5.7, rok: 2023 ; AIS: 0.899, rok: 2023
Způsob publikování: Open access
Web výsledku:
https://pubs.acs.org/doi/10.1021/acs.jafc.4c06771DOI: https://doi.org/10.1021/acs.jafc.4c06771

The preparation of pure metabolites of bioactive compounds, particularly (poly)phenols, is essential for the accurate determination of their pharmacological profiles in vivo. Since the extraction of these metabolites from biological material is tedious and impractical, they can be synthesized enzymatically in vitro by bacterial PAPS-independent aryl sulfotransferases (ASTs). However, only a few ASTs have been studied and used for (poly)phenol sulfation. This study introduces new fully characterized recombinant ASTs selected according to their similarity to the previously characterized ASTs. These enzymes, produced in Escherichia coli, were purified, biochemically characterized, and screened for the sulfation of nine flavonoids and two phenolic acids using p-nitrophenyl sulfate. All tested compounds were proved to be substrates for the new ASTs, with kaempferol and luteolin being the best converted acceptors. ASTs from Desulfofalx alkaliphile (DalAST) and Campylobacter fetus (CfAST) showed the highest efficiency in the sulfation of tested polyphenols. To demonstrate the efficiency of the present sulfation approach, a series of new authentic metabolite standards, regioisomers of kaempferol sulfate, were enzymatically produced, isolated, and structurally characterized.
Trvalý link: https://hdl.handle.net/11104/0357178


Vědecká data: https://pubs.acs.org/